Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis研究代表者

间充质干细胞（Mesenchymal stem cells, MSCs）可分化为多种结缔组织细胞。MSCs在关节软骨修复中的临床应用已有几种方法；然而，与支架材料的选择有关的许多问题，包括其支持细胞活力和分化的能力以及其在原位的保留和降解。通过支架应用间充质干细胞也需要技术上要求很高的外科手术。本研究的目的是检测悬浮在透明质酸（HA）中的间充质干细胞在自发性骨关节炎（OA） Hartley株豚鼠膝关节内关节内移植的结果。移植后5周，HA-MSC组出现部分软骨修复，而其他组没有。检测cfda - se标记的细胞显示HA-MSC组的MSC迁移、分化和增殖。OA软骨中残余软骨细胞和移植间充质干细胞周围均可见较强的II型胶原免疫染色。结论：这种无支架且技术要求低的技术似乎可以在自发性OA动物模型中实现关节软骨的再生。尽管对移植的长期影响进行进一步的研究是必要的，但研究结果表明，关节内注射HA-MSC混合物可能对OA有益。

Mesenchymal stem cells (MSCs) can differentiate into various connective tissue cells. Several techniques have been used for the clinical application of MSCs in articular cartilage repair; however, there are many issues associated with the selection of the scaffold material, including its ability to support cell viability and differentiation and its retention and degradation in situ. The application of MSCs via a scaffold also requires a technically demanding surgical procedure. The aim of this study was to test the outcome of intra-articular transplantation of mesenchymal stem cells suspended in hyaluronic acid (HA) in the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis (OA). At 5 weeks post transplantation, partial cartilage repair was noted in the HA-MSC group but not in the other groups. Examination of CFDA-SE-labeled cells demonstrated migration, differentiation, and proliferation of MSC in the HA-MSC group. There was strong immunostaining for type II collagen around both residual chondrocytes and transplanted MSCs in the OA cartilage. Conclusion: This scaffold-free and technically undemanding technique appears to result in the regeneration of articular cartilage in the spontaneous OA animal model. Although further examination of the long-term effects of transplantation is necessary, the findings suggest that intra-articular injection of HA-MSC mixture is potentially beneficial for OA.