Development of novel therapeutics to inhibit cell proliferation viaPOU6F1 in ovarian clear cell carcinoma

ARID1A是SWI/SNF染色质重塑复合体的一个成员，其成员具有螺旋和atp酶活性，被认为通过改变基因周围的染色质结构来调节某些基因的转录。ARID1A（一种推定的肿瘤抑制蛋白）BAF250a的缺失最近被描述为卵巢透明细胞癌的常见事件。在这项研究中，我们报告了在携带ARID1A突变的卵巢透明细胞癌细胞中恢复野生型ARID1A表达足以抑制细胞增殖并增强抗癌药物敏感性。基因表达分析确定了ARID1A的几个下游靶点。我们的研究结果提供了功能性证据，支持ARID1A是一个真正的肿瘤抑制基因的假设。

ARID1A, a member of SWI/SNF chromatin remodeling complex, whose members have helices and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Loss of the ARID1A (a putative tumor suppressor) protein BAF250a has recently been described as a frequent event in ovarian clear cell carcinoma. In this study, we report that restoring wild-type ARID1A expression in ovarian clear cell carcinoma cells that harbor ARID1A mutations is sufficient to suppress cell proliferation and enhance anticancer agent sensitivity. Gene expression analysis identified several down stream targets of ARID1A. Our results provide functional evidence in support of the hypothesis that ARID1A is a bona fide tumor suppressor gene.