Development of a estimation method for difference of functional sites among splicing isoforms

尽管多种剪接异构体都是通过选择性剪接产生的，但研究剪接异构体功能的剪接异构体比已知的剪接异构体序列数据要少得多。因此，我开发了一种管道，可以估计剪接异构体的功能意义。利用这条管道，我估计了剪接异构体的功能意义，这些剪接异构体失去了对形成疏水核心非常重要的部分，似乎不稳定。结果表明，约有20%的这种剪接异构体具有重要的功能。

Although many kinds of splicing isoforms are produced by alternative splicing, splicing isoforms whose functions are investigated are much fewer than all known sequence data of splicing isoforms. Therefore, I developed a pipeline that can estimate a functional significance of splicing isoforms. Using the pipeline, I estimated the functional significance of splicing isoforms that lost their parts important for forming hydrophobic core and seemed not to be stable. As a result, it is suggested that about 20% of such splicing isoform are functionally significant.