Analysis ofthe relationship between tchoenformational stabilityof phospholipaseA2 and the amount of IgG production.

为了研究磷脂酶A2同工酶（BPII）的构象稳定性与IgG产生量之间的关系，我制备了N-末端区域（氨基酸位置1-8）通过BrCN处理而缺失的去稳定的BPII（BrCN-BPII）。因此，BrCN-BPII不能导致免疫应答的增加。另一方面，我检查了BPII对C2 C12细胞（小鼠成肌细胞系）的细胞死亡活性。当用完整的BPII处理C2 C12细胞时，细胞的活力降低。有趣的是，BrCN-BPII不能诱导细胞死亡。结果表明，BPII的N-末端区域是其细胞死亡的必要元件。

To examin relationship between the conformational stability of phospholipaseA2 isozyme (BPII) and the amount of IgG production, I prepared the destabilized BPII where the N-terminal region (amino acid position 1-8) was deleted by BrCN treatment (BrCN-BPII). As a result, BrCN-BPII could not lead to increase in the immune response. On the other hand, I examined the cell death activity of BPII against C2C12 cells (mouse myoblast cell line). When C2C12 cells were treated with intact BPII, the viability of the cells decreased. Interestingly, BrCN-BPII could not induce the cell death. It was suggested that the N-terminal region of BPII is an essential element for its cell death.