Application to the emergency care by establishment of high-throughput examination systems for transcription factor NF-κB activation

为了提高全身性炎症反应综合征（SIRS）的生存率，我们开发了新的炎症诊断系统。利用真核生物制造的耐热性DNA聚合酶，对细菌的灵敏、可靠的检测在大范围内变得可行，从而使开发广泛的强大应用成为可能。此外，我们利用荧光相关光谱（FCS）建立了一种新的高通量测量系统，通过直接观察溶液中的布朗运动来检测共聚焦区域中荧光标记分子的大小和数量，因为NF-κB活性的升高反映了急性炎症。检测SIRS患者的NF-κB活性可作为紧急护理的炎症标志物。

We developed new inflammation diagnostic system in order to raise the lifesaving rate of systemic inflammatory reactive syndrome, SIRS. Using the eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.Further, we have established a novel high-throughput measurement system for the NF-κB activity using fluorescence correlation spectroscopy （FCS), which was a methodology to examine the size and number of fluorescence-labeled molecules in a confocal area by direct observation of Brownian motion in solutions, because the elevation of NF-κB activity reflects the acute inflammation. Measuring the NF-κB activity in the patients with SIRS would be useful as inflammatory marker for emergency care.