Analysis of human fetal hematopoiesis using human ES cells

我们建立了人胚胎干细胞与来源于人胚胎干细胞的自体基质细胞共培养体系，诱导其向造血/血细胞分化，真正反映人胎儿造血功能。人类胚胎干细胞来源的基质细胞显示出与间充质干细胞（MSCs）同源的表型，并且在与这些人类胚胎干细胞来源的MSCs共培养中发育的造血/血细胞显示来自成人型造血。目前的培养系统使用的是自体人胚胎干细胞。衍生的间充质干细胞可用于分析人类胎儿造血。

We established a coculture system of human ES cells with autologous stromal cells derived from human ES cells to induce their differentiation into hematopoietic/blood cells, truly reflecting human fetal hematopoiesis. The human ES cell-derived stromal cells revealed a homologous phenotype of mesenchymal stem cells(MSCs), and hematopoietic/blood cells developed in the coculture with these human ES cell-derived MSCs were shown to originate from adult-type hematopoiesis. The present culture system using autologous human ES cell. derived MSCs can be useful for the analysis of human fetal hematopoiesis.