Development of biosensing technologies in an optical pickup style

研究了一种创新的生物传感技术，其工作原理是简单地聚焦单个激光束，并以类似于光学拾取单元的方式检测其反射强度。利用聚焦可见光在玻璃-邻苯二胺溶液界面上沉积了聚苯二胺纳米结构。通过背向散射光强度监测聚合物纳米结构的生长。由于生长速度依赖于被氧化的o-Pd对光的吸收，因此背反射振荡曲线可以用来监测过氧化物酶在溶液中的反应。可以在短时间内对葡萄糖进行可靠的光学定量。此外，还研究了基于银纳米颗粒光学捕获的分子检测的表面增强拉曼光谱。用光学捕获法制备了银纳米粒子和分析物分子的聚集体，并将其固定在玻璃衬底上，通过可见激光照射聚集体测量了SERS光谱。

An innovative biosensing technique working by simply focusing a single laser beam and detecting its reflection intensity in a manner similar to the optical pickup unit has been studied. Polyphenylenediamine nanostructures were deposited by a focused visible laser beam on a glass- o-phenylenediamine solution interface. Growth of the polymer nanostructure was monitored by a backscattered light intensity. Because the growth speed depends on light absorption by the oxidized o-PD, the backreflection oscillation curve can be used to monitor the peroxidase enzyme reaction in the solution. A reliable optical quantification of glucose can be performed in a short time. SERS based on molecular detections by using optical trapping of silver nanoparticles were also studied. Aggregates of silver nanoparticles and analyte molecules were produced by optical trapping and fixed on a glass substrate and the SERS spectra were measured by irradiating a visible laser beam on the aggregates.