Molekulare Evolution von Reproduktionsgenen bei Drosophila

该项目的目标是利用最近从果蝇身上获得的基因组资源，对更快的雄性进化假说进行一般测试。具体目标如下：(1)利用现有的黑腹瓢虫基因组序列以及EST和微阵列实验数据，鉴定具有性别特异性或性别富集表达的基因；(2)从黑腹瓢虫种亚群的其他成员中克隆和序列同源基因；(3)根据物种间非同义/同义替代率的比率确定这些基因的分子进化速率，并比较这些基因在雄性、雌性和非偏倚表达下的速率。(4)利用最新发展的种间和种内DNA序列数据统计方法，检验积极选择在生殖基因进化中的可能作用。

The goal of this project is to perform a general test of the faster male evolution hypothesis using recently available genomic resources from Drosophila melanogaster. The specific objectives are as follows: (1) Use the available genome sequence of D. melanogaster and data from EST and microarray experiments to identify genes with sex-specific or sex-enriched expression, (2) clone and sequence orthologs of these genes from other members of the D. melanogaster species subgroup, (3) determine the rate of molecular evolution of these genes based on the ratio of nonsynonymous/synonymous substitution rates among species and compare these rates among genes with male-, female- and non-biased expression, (4) test for the possible role of positive selection in the evolution of reproductive genes using recently developed statistical methods on both inter- and intraspecific DNA sequence data.