Gene expression variation in natural populations of Drosophila

果蝇自然群体的基因表达变异

• 批准号: 86935157
• 负责人: Professor Dr. John Parsch
• 金额: --
• 依托单位: Department Biologie II (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/86935157?language=en
• 关键词: Gene; expression; variation; natural; populations

This project investigates local adaptation at the gene-regulatory level. The main approach is to quantify gene expression variation within and between populations and identify genes that show a large expression difference between populations, but low expression polymorphism within populations. Such genes represent candidates for those that have undergone adaptive regulatory evolution to the local environment. In the first phase of the project, we carried out a microarray analysis to identify genes that differ in expression between an ancestral (African) and a derived (European) population of D. melanogaster. In the second phase, we propose to perform detailed population genetic and functional analyses of the top candidate genes in order to determine the link between genetic variation and expression variation, as well as the effect of expression variation on the organism’s phenotype. These studies will allow us to determine the precise genetic target of selection, as well as the phenotype that is under selection.

这个项目在基因调控层面上研究局部适应。主要方法是量化种群内和种群间的基因表达差异，并找出种群间表达差异较大但种群内表达多态较低的基因。这些基因代表了那些已经经历了适应当地环境的调节进化的候选基因。在该项目的第一阶段，我们进行了微阵列分析，以确定黑腹毛虫祖先(非洲)和衍生(欧洲)种群之间表达差异的基因。在第二阶段，我们建议对主要候选基因进行详细的群体遗传学和功能分析，以确定遗传变异和表达变异之间的联系，以及表达变异对生物体表型的影响。这些研究将使我们能够确定选择的确切遗传目标，以及正在选择的表型。

项目成果

Adaptive divergence of a transcriptional enhancer between populations of Drosophila melanogaster

• DOI: 10.1098/rstb.2013.0024
• 发表时间: 2013-12-19
• 期刊: PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
• 影响因子: 6.3
• 作者: Glaser-Schmitt, Amanda;Catalan, Ana;Parsch, John
• 通讯作者: Parsch, John