4-Laser Cell Sorter

适体形式的功能核酸在研究、诊断和治疗中正变得越来越重要。在迈耶研究小组中，跨学科项目专注于开发DNA和RNA适配子，这些适配子选择性地将目标分子结合在细胞上和细胞内。例如，这些适配子用于细胞RNA的光遗传控制，检测细胞内的代谢物，或将其他分子定向运输到细胞。传统的体外选择方法(SELEX)是基于适体与靶分子的结合，但不允许直接选择适体在细胞上或细胞内所需的功能。有了所要求的细胞分选器，正在建立新的选择方法，使功能适配子能够对靶结构进行分选，从而使这一过程更加简化和快速。此外，使用细胞分类器在相关的蜂窝环境中重新分析和优化现有适配子的性质。功能适配子候选的分选基于一个或多个荧光信号。根据它们随后的使用，样品被收集在反应管、微孔板或载玻片上。

Functional nucleic acids in the form of aptamers are gaining increasing importance in research, diagnostics, and therapy. In the Mayer research group, interdisciplinary projects focus on developing DNA and RNA aptamers that selectively bind target molecules on and within cells. These aptamers are used, for instance, for optogenetic control of cellular RNA, detection of metabolites within the cell, or targeted transport of other molecules to the cell. Conventional in vitro selection methods (SELEX) are based on the binding of aptamers to target molecules but do not allow direct selection for the desired function of aptamers on or within cells. With the requested cell sorter, novel selection methods are being established that enable the sorting of functional aptamers to target structures, making the process more streamlined and expedited. Furthermore, the properties of existing aptamers are re-analyzed and optimized within the relevant cellular environment using the cell sorter. Sorting of functional aptamer candidates is based on one or more fluorescence signals. Depending on their subsequent use, the samples are collected in reaction tubes, microplate wells, or onto slides.