Architecture of the Central Nervous System in Nemathelminthes

该项目希望在免疫组织染色技术和共聚焦激光扫描显微镜的基础上，提供关于Nemathelminthes代表的神经系统的详细信息。前两个阶段产生了关于单个染色的信息内容的基本信息，涉及整个神经系统，以及与具有角质层的动物一起工作的方法学工具。在纳入该项目的5个分类群中，有3个的数据收集工作取得了成功。调查了天麻属26种，涵盖了整个天麻属的多样性范围。在第三个项目阶段，应该填补一些空白（例如鳞翅目的代表），并对现有数据进行系统发育分析。调查了两种Priapulida，并收集了两种，将在项目二期剩余时间和三期剩余时间进行部分调查。研究人员获得了两种海雀的发育数据，并计划对另一种海雀进行第三阶段的研究。线虫幼虫染色不成功，但现在可以控制材料的获取，并且染色程序将得到优化。在第三阶段，成人也将被调查。采集不成功，但应重复采集。在项目的第三阶段，应该完成物种的收集和调查。同时，将进行联合分析。SPP内研究神经系统的小组目前正在编制一个术语表。在第三阶段，将组装和分析数据矩阵，并提供形态学数据和在SPP中获得的分子数据。

The project wants to provide detailed information on the nervous system of representatives ofthe Nemathelminthes based on immunohistological staining techniques and confocal laser scanning microscopy. The first two phases produced basic information on the information content of single stainings in reference to the entire nervous system as well as methodological tools to work with animals having a cuticle. Data collection has been successful in three of the five taxa included into the project. 26 species of Gastrotricha were investigated, spanning the entire range of gastrotrich diversity. In the third project phase, few gaps (such as representatives of Lepidodasyidae) should be filled and the available data will be phylogenetically analysed. Two species of Priapulida were investigated and two further ones were collected, which will be investigated partly in the remaining time in the second phase of the project and partly in the third phase. Data for two kinorhynch species were obtained, with developmental data and a further species being scheduled for the third phase. Staining of nematomorph larvae was unsuccessful, but now controlled access to material is possible and the staining procedure will be optimized. In the third phase, adults will also be investigated. The collection of Loricifera was unsuccessful, but the collection attempt should be repeated. Within the third phase of the project, the collection and investigation of the species should be finalized. In parallel, joint analyses will be made. The groups working on nervous systems within the SPP are currently working on a glossary. In phase 3, a data matrix will be assembled and analyzed and morphological data are made available for an analysis together with molecular data gained within the SPP.