Central project

本课课组将重点分析植物液泡在低温和渗透胁迫下的动态储存功能。由于我们代表了一个错位的研究人员联盟，中央服务项目必须提供在高度控制的条件下生长的植物材料和遗传物质。此外，中央项目将为所有小组提供各自项目所需的各种植物样品。此外，凯泽斯劳滕的实验室还配备了分析设备，可以对与该联盟相关的所有溶质进行鉴定和定量，例如糖、氨基酸等初级代谢物、低丰度代谢物和硝酸盐、硫酸盐、磷酸盐和所有金属等营养物质（通过ICAP）。我们申请一名兼职技术员（E6/2），他将负责受控条件下的植物生长，突变体植物的繁殖和杂交（包括所需的遗传分析），样品制备和溶质定量，以及液泡纯化和随后的膜制备。需要支持中央协调费用，用于(i)即将举行的两年一次的定期科学会议，（ii）参与实验室之间进行实验的同事交流，（iii）至少两个研讨会的资金，（iv）微阵列分析，(v)国际科学会议的规划和组织，（vi）整个研究小组的旅费和（vii）出版费用。

This research group will focus on the analysis of processes involved and governing the dynamic storage functions of plant vacuoles during cold and osmotic stress. Since we represent a dislocated consortium of researchers the central service project has to provide plant material grown under highly controlled conditions and genetic material. Moreover, the central project will support all groups with various types of plant samples required for their individual projects. In addition, the laboratory in Kaiserslautern is well equipped with analytical devices allowing identification and quantification of all solutes with relevance to the consortium, e.g. primary metabolites like sugars, amino acids, low abundant metabolites and nutrients like nitrate, sulphate, phosphate and all metals (via ICAP). We apply for a half time position for a technician (E6/2) who will be responsible for plant growth under controlled conditions, for mutant plant propagation and crossing (including the required genetic analysis), for sample preparation and solute quantification, as well as for vacuole purification and subsequent membrane preparation. Support for central coordination costs is required for (i) upcoming regular biannual scientific meetings, (ii) exchange of co-workers among the participating laboratories with respect to running experiments, (iii) financing of at least two workshops, (iv) microarray analyses, (v) planning and organisation of an international scientific meeting, (vi) travel money for the whole research group and (vii) publication costs.