MOLECULAR MAPPING OF TIGHT JUNCTION PROTEINS WITH RECOMBINANT 7H6 POLYPEPTIDE

紧密连接位于并置上皮和一类内皮的最上部。紧密连接作为细胞旁通路的屏障，调节离子、小分子和细胞的运输。在本项目中，我们研究了紧密连接蛋白的分子结构以及这些蛋白在体内和体外正常和病变组织中的表达。为了进行这些研究，我们克隆大鼠occludin和产生多克隆抗体对barmotin和occludin。由于barmotin被认为是SMC机械化学运动蛋白，我们在人和大鼠中鉴定了另外四种SMC蛋白。以人结肠癌为研究对象，发现紧密连接蛋白异常表达。在细菌脂多糖处理后，在小鼠肠细胞系中也观察到紧密连接蛋白的失调模式。总之，这些观察结果表明，紧密连接蛋白在正常和疾病组织中起着重要作用。目前的研究已经引出了紧密连接蛋白在各种条件下的作用。

Tight junctions localize uppermost of apposing epithelium and a class of endothelium. Tight junctions serve as a barrier for paracellular pathway and regulate traffics of ion, small molecules and cells. In the present projects, we studied molecular structure of tight junction proteins and those expression in normal and disordered tissues both in vivo and in vitro. To perform these studies, we cloned rat occludin and produced polyclonal antibodies against barmotin and occludin. As barmotin has been suggested to belong SMC mechanochemical motor protein, we identified four more SMC proteins in human and rats. Study using human colon cancer revealed that abberant expression of tight junction proteins were observed. The pattern of dysregulation of tight junction proteins were also observed in mouse intestinal cell lines upon treatment of bacterial lipopolysaccharide. Taken together, these observations suggested that tight junction proteins play an important role in both normal and disordered tissues. The current study has elicited the role of tight junction proteins in various conditions.