Effects of distance training camp on reactive oxygen species (ROS) generation of neutrophils activity in long distance runners.

本研究的目的是研究远距离训练营中性粒细胞活性氧（ROS）生成的活性。在知情同意的情况下，招募了11名男性和7名女性长跑运动员作为参与者。在长距离训练营中，男跑者采集了4次血样，女跑者也采集了4次血样；训练营期间2次，恢复期2次（远程训练营后3天和20天）。利用lucigenin和luminol依赖性化学发光（分别为LgCL和LmCL）测量ROS生成活性的变化。通过LgCL和LmCL的峰高（光子/秒；PH）估计ROS代数。在整个训练营期间，男性和女性跑步者的肌酸磷酸激酶（CPK）活性逐渐增加。特别是在耐力训练营结束后，每个跑步者的CPK活性都显著增加（p<0.05）。在男跑耐力训练营期间，LgCL和LmCL的PH值均略有下降。然而，各PH值未见明显变化。长距离训练营结束后，女性跑者LgCL和LmCL的PH值显著降低（p<0.05）。在距离训练营结束后3天，女性跑者的运动能力显著提高，约为静息水平的3倍（p<0.05）。此外，研究人员还注意到，高水平的活性氧活性一直保持到恢复期的20天。这些结果表明，伴随距离训练的过度应激暂时限制了中性粒细胞活性的ROS生成。综上所述，为了防止过度训练带来的过度压力，在规划一个像远程训练这样的营地计划时，不仅要考虑训练计划，还要考虑食物摄入、充足的睡眠和休息。

The aim of this study was to examine the reactive oxygen species (ROS) generation of neutrophils activity during distance training camp. Eleven male and seven female long distance runners were recruited as participants with informed consent. Blood samples of the male runners were collected four times during the distance training camp, and those of female runners were also collected four times ; twice during the camp period and twice in the recovery period (at 3 and 20 days after the distance training camp). The changes in ROS generating activity were measured using lucigenin - and luminol - dependent chemiluminescence (LgCL and LmCL, respectively). ROS generations were estimated by peak height (photon/sec ; PH) of LgCL and LmCL.Creatine phosphokinase (CPK) activities in male and female runners were increased gradually throughout the duration of the training camp. In particular, after the endurance training camp, the significant increases (p<0.05) in CPK activity were found in each runner. During the endurance training camp of the male runners, the PH of both LgCL and LmCL showed a slight decline. However, no significant changes in each PH were found. Also, the PH of LgCL and LmCL in female runners decreased significantly after the distance training camp (p<0.05). At 3 days after the distance training camp, those of the female runners increased significantly about three-fold of resting level (p<0.05). As well, it was noted that the higher levels of ROS activity were maintained until 20 days of the recovery period.These results suggest that excessive stress accompanying distance training restricts the ROS generation of neutrophils activity temporarily. In conclusion, to prevent excessive stress by overtraining, when planning a camp program such as distance training, it is necessary to consider not only the training program but also foods intake, adequate sleep and rest.