EXAMINATION OF TELOMERASE ACTIVITY AND TUMOR SUPPRESSOR GENE ON HUMAN ORAL CANCERS

本研究的目的是研究抗癌药物对端粒酶的影响。有报道称，癌细胞表达端粒酶，使端粒延长，因此可以无休止地进行细胞分裂。另一方面，顺铂是每个地区最常见的抗癌药物，在本研究中使用。在口腔恶性肿瘤患者的临床标本中，高表达。其中约80%的患者端粒酶活性较高。用子宫癌细胞株A431及其耐药细胞株(C-1、C-2)进行的体外实验中，耐药细胞株的端粒酶活性明显高于原始细胞系，而顺铂对这些裸鼠背部移植瘤的体内实验表明，A431组肿瘤体积缩小。C-1组和C-2组肿瘤体积增大，顺铂对C-1和C-2细胞株无明显作用。A431组端粒酶活性受到抑制，而C-1和C-2组未见端粒酶活性抑制。在体内和体外进行的相同研究得出了类似的结果。结果表明，顺铂敏感性与端粒酶活性之间存在一定的相关性，提示端粒酶活性可作为顺铂敏感性的指标。

The purpose of this study is to examine what influence anticancer drugs will exercise on telomerase. It has been reported that cancer cells can report cell division endlessly since they express telomerase which makes telomera lengthen. On the other hand, cisplatin, which is the most frequent anticancer drug in every region, was used in this study. in clinical specimens from oral malignant tumor patients, the high. value of telomerase activity were shown in approximate 80% of them. In the in vitro experiment which was used the uterine cancer cell line, A431 and it's resistant cell lines (C-1, C-2), the value of telomerase activity in the resistant cell lines showed higher than that in the original cell line, And in the in vivo experiment which cisplatin was administered to these cell lines tumors made in the back of nude mouse, Group A431 was recognized the decrease of tumors volume. However, cisplatin was ineffective against C-1 and C-2 cell lines for group C-1and C-2 increased tumors volume. On the other hand, inhibition of telomerase activity was observed in group A431, but no telomerase inhibition in group C-1 and C-2. The same studies performed in in vivo and in vitro gave similar results. The result was that there existed a definite correlation between cisplatin sensitivity and telomerase activity, In this study, it is suggested that telomerase activity is useful in the index of cisplatin sensitivity.