Forensic applications of a new multiplex analysis on STRs using multi-fluorescently labeled primers

利用一种简便的血痕DNA提取试剂盒，设计了一种荧光标记的3个STR基因座的准确、快速的分型系统，并于1997年发表在《日本法医学杂志》上。我们与PE应用生物系统公司合作，使用商品化的试剂盒AmpFlSTR Profiler Kit，在日语206中建立了9个STR(D3S1358、VWA、FGA、TH01、TPOX、CSF1PO、D5S818、D13S317、D7S820)的数据库。该试剂盒可以用多色荧光染色的引物进行多重聚合酶链式反应，从而对9种STR进行分型。我们计算了9个STR基因座的等位基因频率和用于个人识别和亲子鉴定的统计值。这项工作的结果于1997年在奥斯陆举行的第17届国际法医血液遗传学会上公布，并于1999年发表在《法医科学杂志》上。1998年，我们在13个…基因座、9个上述基因座和另外4个基因座(D18S51、D21S11、D8 STR)上统计了2 0 7名日本人的等位基因频率更多S1179、D16S539)，以及AmpFlSTR Profiler PLUS套件和COFILER套件。鉴于目前外国人，尤其是亚洲人偶尔会关注日本的犯罪行为，我们还调查了118名中国人(北京地区)、122名缅甸人和110名韩国人这13个STR基因座的等位基因频率。结果表明，这个由13个STR组成的系统在每个群体中也具有极高的识别率，每个群体的几个STR基因座在每个座位上的等位基因分布与日本人显著不同。此外，使用9个STR基因座上的日本等位基因频率对Hardy-Weinberg平衡的各种检验进行了评估，结果被总结并发表在DNA多态上。我们应用AmpFlSTR Profiler试剂盒进行了亲子鉴定，即所谓的孤立排除案例，在15个被检测的基因座中，只有一个基因座排除了生物母本食品(在这种情况下是D3S1358)。通过对D1S8和D7S21的MVR-PCR分析，证实了D3S1358的独占等位基因来源于父本的突变。这项工作发表在第82届日本法医学会年会上。我们还将该系统应用于腐烂身体的个人识别，以他们在房子里保存的牙刷作为参考样本。较少

A accurate and rapid typing system at three STR loci with fluorescently labeled primers was devised using a convenient DNA extraction kit from bloodstains, and this work was published in the Japanese Journal of Legal Medicine in 1997. In collaboration with a company, PE Applied Biosystem, we made a database of 9 STRs (D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317, D7S820) in 206 of Japanese with the commercially available kit, AmpFlSTR Profiler kit. This kit makes it possible to type 9 STRs by multiplex PCR using multi-colored fluorescent dyed primers. We calculated allele frequencies and some statistical values for personal identification and paternity testing at 9 STR loci. The result of this work was presented in the 17th International Society of Forensic Haemogenetics in Oslo in 1997, and published in Journal of Forensic Sciences in 1999. In 1998, we calculated allele frequencies in 207 of Japanese at 13 STR loci, 9 loci mentioned above and other 4 loci (D18S51, D21S11, D8 … More S1179, D16S539), with the AmpFlSTR Profiler plus kit and the Cofiler kit. Since foreigners, especially Asian populations are occasionally concerned the crimes in Japan currently, we also investigated allele frequencies at those 13 STR loci in 118 of Chinese (Beijing area), 122 of Burmese and 110 of Korean. As the result of this work, it was suggested this system of 13 STRs has also extremely high power of discrimination in each population, and each population has a few STR loci significantly different from Japanese in pairwise comparison of allele distribution at each loci. Furthermore, various tests for Hardy-Weinberg equilibrium were evaluated using Japanese allele frequencies at 9 STR loci, and the results were summarized and published in DNA Polymorphisms.We applied the AmpFlSTR Profiler kit to a paternity testing, so-called an isolated exclusion case, in which the biological fatherfood was excluded at only one locus (D3S1358 in this case) in 15 loci examined. It was confirmed that the exclusive allele at D3S1358 was derived from paternal mutantion using MVR-PCR at D1S8 and D7S21. This work was presented in the 82nd annual meeting of Japanese Society of Legal Medicine. We also applied this system to the personal identification of decomposed cadavers using their toothbrushes kept in houses as reference samples. Less