Influence of oral tolerance of food antigens on systemic humoral immune response against vaccinal antigen

鸟类对口服耐受性的认识很少。配方中动物蛋白对疫苗抗原特异性免疫应答的影响尚未见报道。疫苗中涉及的动物蛋白对疫苗免疫应答的影响在鸡中也从未被研究过。本研究旨在阐明1.口服诱导的动物蛋白抗原(APA)在对APA的全身体液免疫应答中是否建立了耐受性；2.在对不同的特异性抗原(DSA)的全身体液免疫应答中是否建立了对口服诱导的APA的旁观者抑制；3.在已经引起对APA的全身体液免疫应答的动物中，APA的旁观者抑制是否成立。免疫耐受雏鸡对APA的体液免疫应答略有下降。静脉注射APA和DAPA后，对DSA的体液免疫应答中也存在旁观者抑制现象。然而，在已经对DSA产生全身体液免疫反应的动物中，在静脉注射APA和DSA后没有发现旁观者抑制。全身性体液免疫反应增强。在这些实验完成后，这些研究的结果将立即公布。对肠道菌群调节和通过口服耐受或旁观者抑制更有效的疫苗接种的进一步研究正在进行中。

Oral tolerance have little clarified in birds. Influence of animal proteins in formula on specific immune responses to vaccinal antigens have never reported. Effects of animal proteins involved in vaccines on the immune response to vaccinal antigen have also never investigated in chickens.This research aims at clarifing 1.whether the tolerance of orally induced animal protein antigen (APA) is established or not in the systemic humoral immune response to the APA, 2.whether the bystander suppression of orally induced APA is established or not in the systemic humoral immune response to the different special antigen (DSA), and 3.whether the bystander suppression of APA is established or not in animals already raised systemic humoral immune response against APA.As a result, the oral tolerance of APA was induced after long term oral administration of APA. The systemic humoral immune response against APA was slightly reduced in the tolerance-induced chickens. The bystander suppression was also established in systemic humoral immune response to DSA after intravenous induction of both APA and dAPA. In animals already raised systemic humoral immune response to DSA, however, the bystander suppression was not found after intravenous induction of both APA and DSA. The systemic humoral immune response was enhanced.On the completion of these experiments, the results obtained in these researches will be immediately published. Further investigations on the regulation of intestinal flora and the more effective vaccination by means of the oral tolerance or the bystander suppression are proceeding.