Safer gene editing tools for Australian livestock and biotech industries

以有效和安全的方式编辑生物体的基因组对于畜牧业和生物技术行业至关重要。虽然CRISPR-Cas9已成为基因组编辑的首选方法，但已知它会引入不需要的“中靶”和“脱靶”突变，限制了其实用性。为了解决这个问题，CI团队创建了一种名为Crackling-CAST的新型基因组编辑平台技术，该技术几乎100%准确，同时保留了经典Cas9系统的效率。该项目将在生物技术和畜牧业使用的细胞类型中验证新型基因靶向平台的能力，确保这些行业在全球范围内采用该平台，并为澳大利亚带来重大的经济效益。

Editing the genome of an organism in an efficient and safe fashion is critical for the livestock and biotechnology industries. While CRISPR-Cas9 has become the method of choice for genome editing, it is known to introduce unwanted "on-target" and "off-target" mutations, limiting its utility. To address this the CI team created a novel genome editing platform technology termed Crackling-CAST that is almost 100% accurate, while retaining the efficiency of the classical Cas9 system. This project will exemplify the capabilities of the novel gene targeting platform in cell types used by the biotechnology and livestock sectors, ensuring its global uptake by these industries and delivering significant economic benefits for Australia.