Husbandry and Management Core

项目摘要： 维持非人类灵长类动物SPF繁殖群需要几个因素来确保动物的 身体和行为健康、繁殖生产、遗传多样性和特定病原体的排除。的 猪尾猕猴M. nemestrina表现出额外的身体和行为特征 菌落维持的额外挑战。M.线虫属对病原体表现出更高的易感性 许多微生物的影响。它们对慢病毒的易感性使它们成为了研究的有价值的动物模型。 HIV/AIDS研究。1 -11然而，在标准户外繁殖群体中的非人灵长类动物暴露于 环境和媒介传播的病原体，如克氏锥虫和犬球孢子菌，以及M. 线虫属在这种暴露下表现出对疾病易感性增强。还必须注意 防止M。婴儿体温过低。与恒河猴（M. mulatta），其表现出 季节性繁殖，婴儿M。nemestrina全年出生，必须保持在一个加热的 环境 在繁殖管理上，M. mulatta和 M.奈美丝蒂娜M.黑白混血儿可以在 大型畜栏中的大型多雄多雌群体，基础设施最少。相比之下，M。nemestrina 在野生环境中形成单雄多雌的分裂/融合群体12，13，并试图形成多雄群体 从而导致严重创伤。即使是稳定的单雄繁殖群体，M的生产力也较低。 nemestrina比M. mulatta.14华盛顿国家灵长类动物研究中心（WaNPRC）拥有超过50个 年的育种经验，15和有专业知识和资源，以满足这些 挑战 有了这份U42合作协议的支持，我们将确保SPF M的健康。线虫群落， 包括保持动物不感染B病毒、SIV、SRV和STLV，沿着控制环境 微生物，例如T. cruzi和C.免疫球蛋白利用适当的生物信息学工具，我们将提高产量 的SPF M。以符合成本效益的方式满足艾滋病毒/艾滋病调查人员的需要。我们将提供 SPF M。根据NIH指导的优先级，向研究者提供nemestrina。我们将扩大预分配 对动物进行T.克氏病毒、黄病毒、CMV和AAV。

Project Summary: Maintenance of a non-human primate SPF breeding colony requires several factors to ensure the animals' physical and behavior health, breeding production, genetic diversity, and exclusion of specified pathogens. The pigtailed macaque, M. nemestrina, exhibits additional physical and behavioral characteristics that pose additional challenges in colony maintenance. M. nemestrina exhibit greater susceptibility to the pathogenic effects of many microorganisms. Their susceptibility to lentiviruses makes them valuable animal models for HIV/AIDS research.1-11 However, nonhuman primates in standard outdoor breeding colonies are exposed to environmental and vector-borne pathogens, such as Trypanosoma cruzi and Coccidioides immitis, and M. nemestrina demonstrate enhanced susceptibility to disease with such exposure. Care must also be taken to prevent M. nemestrina infants from hypothermia. In contrast to rhesus macaques (M. mulatta) which exhibit seasonal breeding, infant M. nemestrina are born year-round and must be maintained in a heated environment. Breeding management is also different between M. mulatta and M. nemestrina. M. mulatta can be bred in large multi-male, multi-female groups in large corrals with minimal infrastructure. In contrast, M. nemestrina form single male multi-female fission/fusion groups in the wild12,13 and attempts to form multi-male groups result in significant trauma. Even with stable, single-male breeding groups, productivity is lower for M. nemestrina than for M. mulatta.14 The Washington National Primate Research Center (WaNPRC) has over 50 years of breeding experience with this species,15 and has the expertise and resources to meet these challenges. With support from this U42 cooperative agreement, we will ensure the health of the SPF M. nemestrina colony, including maintaining the animals free of B virus, SIV, SRV, and STLV, along with control of environmental organisms, such as T. cruzi and C. immitis. Using appropriate bioinformatics tools, we will increase production of SPF M. nemestrina to meet the needs of HIV/AIDS investigators in a cost-effective manner. We will provide SPF M. nemestrina to investigators in accordance with NIH-directed priorities. We will expand pre-assignment screening of animals for T. cruzi, flaviruses, CMV, and AAV.