Disclipine A: Microbiology - Analytical Track Animal Food Product and Environmental Testing

摘要 项目二：德克萨斯州动物食品和微生物环境测试 危害 德克萨斯州化学家办公室（OTSC）有能力筛选和/或确认以下内容 微生物：沙门氏菌属动物食品、动物食品配料、生物固体、人类食品; 动物性食品中的产滋贺样毒素大肠杆菌（STEC）O 157：H7和非O 157，动物性食品 原料和人类食品;生奶、动物食品和动物食品中的单核细胞增生李斯特菌 成分，人类食品; SEB在生牛奶，布鲁氏菌属。原料奶中的抗原，蓖麻毒素， Tetracore ELISA，志贺氏菌属在人类食物中，炭疽杆菌在人类食物中存在， 人类食物中的葡萄球菌肠毒素、T-2毒素和α-鹅膏蕈碱（LCMSMS）。的 microlab接受了炭疽杆菌和耶尔森氏菌筛查方法的BSL 3培训 鼠疫在USAMRIID设施，Ft Detrick，MD在2018年，并正在向前迈进，以建立这一 方法（筛选和确认）在OTSC机构。OTSC-AAS微生物学实验室位于 将其微生物学能力纳入OTSC ISO/IEC 17025：2017范围的过程 这将发生在项目启动之前。OTSC动物食品产品和环境 测试，微生物学测试项目计划包括：1）通过OTSC现场采集样本 调查人员使用批准的抽样方法和监管链，2）分析500 沙门氏菌和单核细胞增生李斯特菌样本，以及50个禁用哺乳动物样本 组织，3）1天内报告推定阳性，3天内报告确证结果， 以及4）从市场上清除受污染的产品。作为该项目的成果，德克萨斯州 动物食品微生物危害的监测将得到加强，ISO范围 扩大了认证，提高了实验室的容量和能力， 食品微生物危害将减少风险和产品掺假， 污染物。

Abstract PROJECT TWO: Texas Animal Food Product and Environmental Testing for Microbiological Hazards Office of the Texas State Chemist (OTSC) has the ability to screen and/or confirm the following organisms: Salmonella spp. in animal food, animal food ingredients, biosolids, human food; Shiga like toxin producing E.coli (STEC) O157:H7 and non-O157 in animal food, animal food ingredients and human food; Listeria monocytogenes in raw milk, animal food and animal food ingredients, human food; SEB in raw milk, Brucella spp. antigen in raw milk, ricin toxin via Tetracore ELISA, Shigella spp. in human food, Bacillus anthracis sterne in human food, Staphylococcal enterotoxins in human food, T-2 toxin and alpha- amanitin by LCMSMS. The microlab received BSL3 training in screening methodologies of Bacillus anthracis and Yersinia pestis at USAMRIID facility, Ft Detrick, MD in 2018 and are moving forward to establish this method (screening and confirmatory) at OTSC facility. The OTSC-AAS microbiology lab is in process of bringing its microbiology capabilities under the OTSC ISO/IEC 17025:2017 scope which will occur prior to project startup. The OTSC Animal Food Product and Environmental Testing, Microbiology testing project plan includes: 1) collecting samples by OTSC field investigators using approved sampling methodology and chain-of-custody, 2) analyzing 500 samples for Salmonella and Listeria monocytogenes, and 50 samples for prohibited mammalian tissue, 3) reporting presumptive positives within 1 day and confirmatory results within 3 days, and 4) removal of contaminated product from the market. As an outcome of this project, Texas animal food surveillance of microbiological hazards will be strengthened, scope of ISO accreditation expanded, laboratory capacity and capability increased, and surveillance of animal food for microbiological hazards will reduce risk and product adulteration from these contaminants.