Project 3 - Ex vivo immune profiling of dengue viruses and vaccines

通过比较分析，不同的冠状病毒，包括新型SARS-CoV-2和 人类季节性冠状病毒hCoV-OC43和hCoV-NL63将用于揭示其 细胞易感性和易感性、先天免疫的异同 包括单核细胞在内的原代人类细胞系统的反应和免疫调节潜力 来源的树突状细胞(MDDC)，正常人的支气管上皮细胞(NHBE)，以及一种 活体扁桃体组织培养系统。我们将使用最先进的技术来分析这些 细胞内和细胞外的反应。我们的团队专门研究操纵 原代人类细胞系统和研究对病毒感染的先天免疫反应 以及先天免疫感应的病毒拮抗作用。因此，我们的定位是独特的 应对这一新的公共卫生威胁并提供有关分子的关键信息 SARS-CoV-2的生物学。

Through comparative analysis, different coronaviruses, including the novel SARS-CoV-2 and human seasonal coronaviruses hCoV-OC43 and hCoV-NL63 will be used to unveil both their similarities and differences in cellular susceptibility and permissiveness, innate immune responses, and immune modulatory potential in primary human cell systems including monocyte derived dendritic cells (MDDCs), normal human bronchial epithelial cells (NHBEs), and an ex vivo tonsil histoculture (HC) system. We will use state of the art techniques to analyze those responses intracellularly and extracellularly. Our group specializes in the manipulation of primary human cell systems and investigating both innate immune responses to viral infection as well as viral antagonism of innate immune sensing. As such we are uniquely positioned to respond to this new public health threat and provide critical information regarding the molecular biology of SARS-CoV-2.