Histology

组织学核心：项目摘要/摘要 科赫研究所成员使用脊椎动物模型生物作为关键工具来研究已知或假定的 癌症基因与肿瘤的发生发展；研究肿瘤发生、发展的机制 和转移；评估间质和免疫反应在肿瘤发生中的作用；评估疗效 药物、纳米材料和设备在治疗和诊断方面的应用。因此，至关重要的是中心 成员能够正确诊断发育和肿瘤的表型，评估潜在的 分子事件，并准确和定量地评估药物或疫苗递送，局部组织反应， 和总的治疗反应。科赫研究所组织学核心是一个共享资源，提供国家- 最先进的组织学服务来支持这些研究。这包括组织方面的援助和/或培训 切片、幻灯片准备、染色和分析，以及获得咨询服务 国际公认的兽医病理学家罗德里克·布朗森博士，用于诊断肿瘤和组织 表型。 在本期间，组织核心的使用率从67%增加到中心成员的77%，以及 包括来自所有三个项目的调查人员。为了回应中心成员的需求，组织学核心 扩展服务和工具。这包括扩大免疫组织化学和 特殊污渍，以及针对独立切片的调查人员培训。Core还获得了新的数字幻灯片 更新了扫描能力和量化软件，并升级了现有的关键仪器。因此， 这种共享资源对于科赫研究所任务的成功至关重要。 在即将到来的时期，组织学核心致力于提供广泛的最先进的组织学 为中心成员的研究项目提供支持的服务。计划的举措包括：与 整合基因组学和生物信息学核心，建立基因表达图谱的方法学 组织内的单细胞水平；开发专业知识并提供使用自动化人工智能驱动的指导 用于组织切片分析的分析包(如QuPath)；评估仪器以 确定荧光数字幻灯片扫描的社区效益；并将组织学培训课程扩展到 包括更多的实践研讨会机会。此共享核心对CCSG具有非凡的价值，因为 科赫学会会员占核心服务使用量的95%。值得注意的是，请求的CCSG#年预算 49年比本期(48年)预算减少6.8%。

Histology Core: Project Summary/Abstract Koch Institute Members use vertebrate model organisms as a key tool to study the role of known or putative cancer genes in development and tumorigenicity; investigate the mechanisms of tumor initiation, progression and metastasis; evaluate the role of stroma and immune responses in tumorigenesis; and assess the efficacy of drugs, nanomaterials and devices in therapeutic and diagnostic applications. Thus, it is essential that Center Members are able to correctly diagnose developmental and tumor phenotypes, evaluate the underlying molecular events, and accurately and quantitatively assess drug or vaccine delivery, localized tissue response, and overall therapeutic response. The Koch Institute Histology Core is a Shared Resource that provides state- of-the-art histological services to support these studies. This includes assistance and/or training in tissue sectioning, slide preparation, staining and analysis, and access to the consultative services of an internationally recognized Veterinary Pathologist, Dr. Roderick Bronson, for diagnosis of tumor and tissue phenotypes. During the current period, usage of the Histology Core increased from 67% to 77% of Center Members, and included investigators from all three Programs. In response to Center member needs, the Histology Core expanded services and instrumentation. This includes expanded offerings for immunohistochemistry and special stains, and investigator training for independent sectioning. The Core also acquired new digital slide scanning capabilities and quantification software, and made upgrades to existing key instrumentation. Thus, this Shared Resource is essential to the success of the Koch Institute mission. In the upcoming period, The Histology Core is committed to offering a wide range of state-of-the-art histological services to support the research programs of Center Members. Planned initiatives include: working with the Integrated Genomics & Bioinformatics Core to establish methodologies for mapping gene expression at the single cell level within tissues; developing expertise and providing guidance in use of automated AI-driven analysis packages (such as QuPath) for analysis of histological sections; evaluating instrumentation to determine community benefit for fluorescent digital slide scanning; and expanding histology training offerings to include more hands-on workshop opportunities. This shared Core is of exceptional value to the CCSG because Koch Institute Members account for 95% of the Core services usage. Notably, the requested CCSG budget for Year 49 is 6.8% less than the budget in the current period (Year 48).