Flow Cytometry

摘要-流式细胞术共享资源 流式细胞术共享资源为癌症中心成员提供多参数评估和高 加速表型不同细胞群的富集。Core提供全业务高速小区 由专业操作员利用生物安全外壳中的两台BD Aria仪器进行分类。用于分析 细胞计数，研究者可以选择由核心工作人员进行全方位服务分析或接受培训，然后 独立地使用六个分析流式细胞仪中的一个。这些分析型细胞仪，全天候提供给受过培训的 用户，包括两个BD Fortessas，其中较新的是一个5激光，18色仪器，和一个ImageStreamX MkII成像流式细胞仪，配有4个激光器和12个成像通道。许多地核的血细胞计数器 自动装载机，使更大规模的实验。分析软件可在核心或通过机构 网站许可证。这一被广泛使用的核心项目由气候变化委员会管理，并得到研究所的支持， 仪器设备采购和维护。在上一个融资期内，三大技术平台分别为 收购，成像流式细胞仪作为一项新的服务推出。核心主任Altman先生和工作人员 在NCI指定的癌症中心流式细胞仪核心方面拥有超过30年的经验， 他们在项目的任何阶段提供全面的技术指导，从实验设计到 手稿准备。流式细胞术核心被广泛使用，因为在过去5年中，有46例患者使用了它。 癌症中心实验室代表所有三个项目，核心贡献了至少75个癌症相关的 出版物。

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource offers Cancer Center members multi-parametric evaluation and high- speed enrichment of phenotypically distinct cell populations. The Core provides full-service high-speed cell sorting performed by expert operators, utilizing two BD Aria instruments in biosafety enclosures. For analytical cytometry, investigators have a choice of either full-service analysis by Core staff or receiving training and then independently using one of six analytical flow cytometers. Those analytical cytometers, available 24/7 to trained users, include two BD Fortessas, the newer of which is a 5-laser, 18-color instrument, and an ImageStreamX MkII imaging flow cytometer with four lasers and 12 imaging channels. Many of the Core's cytometers have autoloaders, enabling larger-scale experiments. Analysis software is available in the Core or via the Institutional site license. This broadly used Core is managed by the CC and receives support from the Institute for instrumentation purchase and maintenance. In the last funding period, three major technology platforms were acquired, and imaging flow cytometry was launched as a new service. The Core Director, Mr. Altman, and staff have combined experience of more than 30 years in NCI-designated Cancer Center Flow Cytometry Cores, and they provide comprehensive technical guidance at any stage of a project, from experimental design through manuscript preparation. The Flow Cytometry Core is broadly used, as in the last 5 years, it was used by 46 Cancer Center labs representing all three programs, and the Core contributed to at least 75 cancer-related publications.