Project-003

摘要 通过比较分析，不同的冠状病毒，包括新型SARS-CoV-2和 人类季节性冠状病毒hCoV-OC 43和hCoV-NL 63将用于揭示它们的 细胞易感性和宽容性的相似性和差异，先天免疫 免疫应答和免疫调节潜能 来源的树突状细胞（MDDC），正常人支气管上皮细胞（NHBE），以及一种前 体内扁桃体组织培养（HC）系统。我们将使用最先进的技术来分析 细胞内和细胞外的反应。我们的组织专门研究 原代人类细胞系统和研究先天性免疫反应病毒感染 以及先天免疫感应的病毒拮抗作用。因此，我们处于独特的地位， 应对这一新的公共卫生威胁，并提供有关分子 SARS-CoV-2生物学

ABSTRACT Through comparative analysis, different coronaviruses, including the novel SARS-CoV-2 and human seasonal coronaviruses hCoV-OC43 and hCoV-NL63 will be used to unveil both their similarities and differences in cellular susceptibility and permissiveness, innate immune responses, and immune modulatory potential in primary human cell systems including monocyte derived dendritic cells (MDDCs), normal human bronchial epithelial cells (NHBEs), and an ex vivo tonsil histoculture (HC) system. We will use state of the art techniques to analyze tose responses intracellularly and extracellularly. Our group specializes in the manipulation of primary human cell systems and investigating both innate immune responses to viral infection as well as viral antagonism of innate immune sensing. As such we are uniquely positioned to respond to this new public health threat and provide critical information regarding the molecular biology of SARS-CoV-2.