Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Tissue Mapping

项目摘要 我们建议在范德比尔特建立生物分子多模式成像中心(BIOMIC 大学作为与人类生物分子图谱计划(HuBMAP)相关的组织测绘中心。 BIOMIC的使命是建立一个集成成像和分子分析技术的平台， 使构建全面的人体组织三维分子图谱成为可能。BIOMIC将 利用质谱学研究中心和国家研究中心的独特资源 范德比尔特大学成像质谱学资源与世界级临床 范德比尔特大学医学中心的环境创造了一种能力，在 当前技术无与伦比的理解水平。BIOMIC的创新方面是 三维成像质谱仪(IMS)与多种体内和体外成像的结合 创造新的分子信息医学成像模式的技术。BIOMIC的应用 肾脏、胰腺和骨骼的特定器官项目的平台将提供一个新的理解范式 这些器官在多个维度上的正常状态，既有分子(例如，脂质、代谢物和 蛋白质)和空间(例如，从整个器官到单个细胞)。作为HuBMAP的参与者，分子图谱 由BIOMIC生成，将分发给世界各地的合作者，以促进生成和测试 关于这些重要器官系统功能的新假说，使我们能够对人类有新的认识 健康和疾病。BIOMIC的使命是打造一个能够高效获取和整合的平台 多模式成像数据集，包括IMS、组织学、多路IHC、磁共振成像和高分辨率 分辨率CT成像用于形成完整的三维分子图谱。这项工作需要 创建和整合三项能力，以1)采购和管理标本，2)准备和缓解 分析前因素，以及3)获取、处理和传播多模式成像和大规模组学 数据。BIOMIC平台将使用三个特定于器官的项目进行优化和测试：骨骼、肾脏和 胰腺。

Project Summary We propose the founding of the BIOmolecular Multimodal Imaging Center (BIOMIC) at Vanderbilt University as a Tissue Mapping Center associated with the Human BioMolecular Atlas Program (HuBMAP). The mission of BIOMIC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3-dimensional molecular atlases of human tissues. BIOMIC will leverage the unique resources of the Mass Spectrometry Research Center and the National Research Resource for Imaging Mass Spectrometry at Vanderbilt University along with the world-class clinical environment of the Vanderbilt University Medical Center to create a capability to characterize human tissues at a level of understanding unrivaled by current technologies. The innovative aspect of BIOMIC is the combination of 3-dimesional imaging mass spectrometry (IMS) with a variety of in vivo and ex vivo imaging technologies to create new modalities of molecularly-informed medical imaging. The application of the BIOMIC platform to organ-specific projects in kidney, pancreas, and bone will provide a new paradigm of understanding the normal state of these organs across multiple dimensions, both molecular (e.g., lipids, metabolites, and proteins) and spatial (e.g., whole organs to single cells). As a HuBMAP participant, the molecular atlases generated by BIOMIC will be disseminated to collaborators worldwide to facilitate the generation and testing of new hypotheses regarding the function of these important organ systems, enabling new insight into human health and disease. The mission of BIOMIC is to create a platform that can efficiently acquire and integrate multimodal imaging data sets, including IMS, histology, multiplex-IHC, magnetic resonance imaging, and high- resolution CT imaging for the formation of comprehensive 3-D molecular atlases. This effort requires the creation and integration of three capabilities to 1) procure and manage specimens, 2) prepare and mitigate pre-analytical factors, and 3) acquire, process, and disseminate multimodal imaging and large-scale omics data. The BIOMIC platform will be optimized and tested using three organ-specific projects: bone, kidney, and pancreas.