ShEEP Request for ImageXpress Micro Confocal High Content Screening System

项目总结/摘要 圣刘易斯的ORD共享设备评估计划（ShEEP）提案的目标是 VA医疗系统是为了获得一个高内涵的共焦显微镜成像系统，能够 获得和处理多个样品（包括活细胞）的高分辨率图像。系统 将由VA资助的多名研究人员及其在两所附属大学的同事共享 圣刘易斯大学医学院和华盛顿大学医学院。两个分支机构 都有和圣刘易斯退伍军人管理局有关的大型研究企业该仪器旨在 支持和加强VA资助的科学家的大型和多样化的群体的研究工作。 要求的仪器是Molecular Devices ImageXpress Micro Confocal High-Content 成像系统和附带的分析软件，包括多个应用程序模块， 加载到分析工作站，旨在支持成像和数据处理需求， 各种各样的调查。所提出的系统被配置为旋转圆盘激光共焦 显微镜，以适应快速成像在多个荧光波长，水浸 目的是允许在减少的暴露时间下增加较厚样品的穿透深度， 用于活体样品成像的环境控制和用于未染色细胞成像的相位对比。 除了需要高含量的成像和分析之外， 支持这一提议的研究人员需要成像和分析类器官。这些成年人的茎 细胞衍生的细胞簇概括了它们所来自的组织的许多特征。 获得，因此现在已被采用在多个线的调查，从个性化 肿瘤化疗微生物致病机理的研究。本提案中的多个项目涉及 癌症生物学、血液流变学、肾脏病学、心脏病学和传染病学是围绕 将类器官成像作为基础发现和转化为治疗的平台， 我们在退伍军人事务部的病人

Project Summary/Abstract The goal of this ORD Shared Equipment Evaluation Program (ShEEP) proposal from the Saint Louis VA Healthcare System is to obtain a high content confocal microscopy imaging system capable of obtaining and processing high resolution images of multiple samples, including live cells. The system will be shared by multiple VA-funded investigators and their colleagues at two affiliated universities Saint Louis University School of Medicine and Washington University School of Medicine. Both affiliates have substantial research enterprises connected to the Saint Louis VA. The instrument is designed to support and enhance the research endeavors of a large and diverse group of VA-funded scientists. The instrumentation requested, is a Molecular Devices ImageXpress Micro Confocal High-Content Imaging System and accompanying analysis software that includes multiple application modules pre- loaded onto an analysis workstation designed to support the imaging and data processing needs for a wide variety of investigations. The proposed system is configured for spinning disc laser confocal microscopy to accommodate rapid imaging at multiple fluorescence wavelengths, water immersion objectives that permit increased depth of penetration for thicker samples at reduced exposure times, environmental control for imaging of live samples and phase contrast for imaging of unstained cells. In addition to the need for high content imaging and analysis, a common theme among the investigators supporting this proposal is the need to image and analyze organoids. These adult stem cell-derived clusters of cells recapitulate many of the features from the tissue from which they were obtained and therefore have now been adopted in multiple lines of investigation from personalized cancer chemotherapy to microbial pathogenesis studies. Multiple projects in this proposal relating cancer biology, rheumatology, nephrology, cardiology and infectious diseases are designed around the imaging of organoids as a platform for basic discovery and translation to therapies that would benefit our patients at the VA.