Analytical Chemistry Core

摘要：核心A 分析核心A是加州大学戴维斯分校超级基金研究中心不可或缺的一部分。方法 是为了检测暴露在环境中的危险物质的生物标志物，并评估 这些物质对人类健康的影响。 核心A提供对解决协作研究问题至关重要的分析支持。老练 仪器设备用于开发阐明所需的层析和光谱方法。 生物标记物。具体地说，核心A提供化合物鉴定和评估化学纯度；鉴定 补救产品、与外源生物相关的潜在配体和目标代谢物(项目1、4 为环境中的生物传感器验证和免疫化学检测提供了参考方法 物质(项目2和3)；并提供代谢组学支持(项目1、4和5)。核心A优惠 对有兴趣获取和解释其他校园服务生成的频谱的调查人员提供帮助 核磁共振和蛋白质组设施等实验室。乐器培训和教育也提供给 调查员与培训核心(核心E)合作。 核心A提供一系列创新服务，重点是分析方法的开发。这个 CORE有3个LC-MS/MS、1个LC-ToF-MS、2个GC-MS和1个加速器质谱仪(AMS)。危险的 物质及其代谢物在人类或动物样本中被测量，并可被检测到高水平 敏感度。例如，AMS的检测下限为~1amol 14C，灵敏度为100000倍 而不是传统的液体闪烁计数器。另一种用于评估有害生物标志物的技术 物质是代谢组学分析，是一种高通量的方法，用于筛选生物体内的代谢物 样本。在这里，一种创新的LC-MS/MS方法被用于筛选87种脂类代谢物，以确定 有害物质对调节血脂的影响。 核心A是加州大学戴维斯分校超级基金中心的重要协作组件，推动超级基金向前发展 研究项目。这一核心推动了评估有害生物标志物所需的分析技术 物质。Core A的研究人员继续致力于扩展代谢组学平台，以便 提供更高的灵敏度和更高的吞吐量。AMS吞吐量也在增加，从而实现了高效 样本分析。通过提供创新和先进的技术，这一核心是研究的一个重要部分 程序。它为围绕有害物质环境污染的问题提供解决方案 并询问这些物质对人类健康构成的风险。

ABSTRACT: CORE A The Analytical Core A functions as an integral part of the Superfund Research Center at UC Davis. Methods are developed to detect biomarkers of exposure to hazardous substances in the environment, and to assess the impact these substances have on human health. Core A provides the analytical support critical to solving collaborative research problems. Sophisticated instrumentation is used to develop chromatographic and spectrometric methods necessary for the elucidation of biomarkers. Specifically, Core A provides compound identification and assesses chemical purity; identifies remediation products, potential ligands associated with xenobiotics, and targeted metabolites (Projects 1, 4 and 5); provides reference methods for biosensor validation and immunochemical detection of environmental substances (Projects 2 and 3); and provides metabolomics support (Projects 1, 4 and 5). Core A offers assistance to investigators interested in obtaining and interpreting spectra generated at other campus service laboratories such as the NMR and proteomic facility. Instrument training and education is also offered to investigators in conjunction with the Training Core (Core E). A range of innovative services is offered by Core A, with emphasis on analytical method development. The Core has 3 LC-MS/MSs, 1 LC-ToF-MS, 2 GC-MSs, and an Accelerator Mass Spectrometer (AMS). Hazardous substances and their metabolites are measured in human or animal samples, and can be detected with high sensitivity. For example, the AMS has a detection limit of ~1 amol 14C, and is 100 000 times more sensitive than the traditional liquid scintillation counter. Another technique used to assess biomarkers of hazardous substances is metabolomics analysis, a high throughput method that screens for metabolites within biological samples. Here, an innovative LC-MS/MS method is used to screen for 87 lipid metabolites to determine the consequence of hazardous substances on regulatory lipids. Core A is a significant collaborative component of the UC Davis Superfund Center, driving forward Superfund research projects. This Core advances the analytical techniques required to assess biomarkers of hazardous substances. Researchers within Core A continue to work on expanding the metabolomics platform in order to provide more sensitivity and higher throughput. The AMS throughput is also increasing, allowing for efficient sample analysis. By providing innovative and advanced technology, this Core is a valued part of the research program. It generates solutions to problems surrounding the environmental contamination of hazardous substances, and interrogates the risk these substances pose on human health.