Animal Phenotyping (Core B)

动物表型核心 工程 摘要/摘要 事实证明，肥胖的啮齿动物模型对于肥胖的研究和NYNORC开展的工作来说是非常有价值的 调查人员。为了满足中心研究人员在评估多分子和 肥胖症代谢的系统生理指标，包括炎症、基因表达以及两者 发抖和非发抖产热，拟议的NYNORC动物表型核心(APC)反映了 动物能量平衡核心(爱因斯坦)的显著扩展和与动物组成部分的整合 脂肪组织核心(哥伦比亚)。自我们上次提交(2015)以来，APC已经处理了10,231个组织 在31项赠款的支持下，对36名NYNORC成员的4023只小鼠/大鼠进行了样本和表型鉴定或手术， 并为NIH资助的5个非NYNORC成员提供了额外的服务。APC提供的服务有 在获得18项新的或延长的赠款方面发挥了重要作用，并在119份出版物中发挥了作用。根据他们的经验 随着APC的核心和对高影响力研究期刊不断增长的数据需求，NYNORC成员的 研究项目的回应是扩大了它们的复杂性和对多重自发的渴望 清醒行为动物新陈代谢和组织靶向方法的测量。相应地，新的 14个NYNORC现有成员和新成员要求的服务，包括持续血糖监测， 肌电图术、体内组织特异性产热作用和脂肪组织分析导致了4个新的和1个 续订了R01授权，并产生了来自17个NYNORC用户的更广泛的&gt；250服务请求 这些服务在下一个供资期间的使用情况。拟议的APC显著地集中和协调 扩大了这一合并核心的技术和研究能力。加入额外的动物 爱因斯坦和哥伦比亚大学校园的资源将显著增加用户和研究基础 整个NYNORC，特别是APC核心的。通过协调应用NYNORC-DETECTED 哥伦比亚大学和爱因斯坦大学的设施、资源和专业知识，拟议的APC核心结合了 摄食行为的复杂定性和定量测量，同时评估 能量消耗、体力活动、体温和可重复的无创性身体成分分析 通过MRI和MicroCT检查。还可以进行一系列新陈代谢手术，包括胃肠搭桥术， 自主神经去神经程序和脂肪组织服务包括：1)量化脂肪细胞 亚群(棕色、白色、米色)，在大小、变异和细胞超微结构方面；2)初级 细胞分离和分析；3)脂肪细胞和脂肪组织巨噬细胞分化；4) 脂肪组织移植。这种扩展的服务范围源于人们日益认识到 我们对肥胖生物学的理解需要同时评估能量摄入和 能源消耗，以及重复确定其对能源平衡的净影响的手段。 单独的动物。

Animal Phenotyping Core PROJECT SUMMARY/ABSTRACT Rodent models of obesity have proven invaluable to the study of obesity and to the work carried out by NYNORC investigators. To meet the growing needs of Center researchers in the evaluation of multiple molecular and systemic physiological indices of metabolism in obesity, including inflammation, gene expression, and both shivering and non-shivering thermogenesis, the proposed NYNORC Animal Phenotyping Core (APC) reflects a significant expansion of the Animal Energy Balance Core (Einstein) and integration with the animal component of the Adipose Tissue Core (Columbia). Since our last submission (2015), the APC has processed 10,231 tissue samples and phenotyped or operated on 4023 mice/rats for 36 NYNORC members supported by 31 grants, and provided additional services to 5 NIH-funded non-NYNORC members. The services offered by APC have been instrumental in obtaining 18 new or renewed grants, and in 119 publications. Based on their experiences with the APC core and ever-increasing data demands of high impact research journals, NYNORC members' research programs have responded by expanding in their sophistication and desire for multiple spontaneous measures of metabolism and tissue-targeting approaches in awake behaving animals. Accordingly, new services requested by 14 existing and new NYNORC members, including continuous blood glucose monitoring, electromyography, in vivo tissue-specific thermogenesis, and adipose tissue analyses have led to 4 new and 1 renewed R01 grant, and have generated >250 service requests from 17 NYNORC users for more extensive uses of these services for the next funded period. The proposed APC concentrates and coordinates significantly expanded technical and research capabilities of this amalgamated Core. The incorporation of additional animal resources at both the Einstein and Columbia campuses, will significantly increase the user and research base of the NYNORC overall, and of the APC Core in particular. By coordinated application of NYNORC-dedicated facilities, and resources and expertise at both Columbia and Einstein, the proposed APC Core combines sophisticated qualitative and quantitative measurements of ingestive behavior with concurrent assessments of energy expenditure, physical activity, body temperature, and repeatable noninvasive body composition analysis by MRI and MicroCT. Also available are a range of metabolic surgeries, including gastrointestinal bypass, autonomic denervation procedures, and adipose tissue services including: 1) quantifying adipocyte subpopulations (brown, white, beige), in terms of size, variation, and cellular ultrastructure; 2) primary cell isolation and analysis; 3) differentiation of adipocytes and adipose tissue macrophages; and 4) adipose tissue transplantation. This expanded array of services stems from the growing realization that our understanding of the biology of obesity requires simultaneous assessment of energy intake and energy expenditure, and of the means to determine their net effects on energy balance repeatedly in individual animals.