Enhancement of molecular capabilities of the TAMU VMTH CML

项目总结 在美国，食源性疾病每年都会导致严重的发病率和死亡率。 沙门氏菌、弯曲杆菌和李斯特菌都会引起人和动物的感染。虽然 人类的食源性疾病通常与食用受污染的食物、宠物有关。 可以是细菌病原体的直接和间接来源。美国食品和药物管理局 (FDA)兽医实验室综合反应网络(VET-LIRN)兽医诊断学 实验室合作协议计划旨在促进人类和动物健康 通过提供科学信息和建设实验室应对日常和紧急情况的能力 对暴发疫情的调查和对动物饲料问题的调查 毒品。疫情的检测需要从不同地理位置收集样本的能力 区域。德克萨斯州有2500万人口，人口仅次于加利福尼亚州，而且 马和牛的数量在全国名列前茅。因此，重要的是理解 食源性疾病在德克萨斯州内动物和人中的动态。这个 德克萨斯农工大学兽医教学医院临床微生物学实验室 目前担任FDA Vet-LIRN实验室。实验室的成员们帮助完成了 发展Vet-LIRN检测方法，包括分子检测，沙门氏菌和 空肠弯曲杆菌，参加能力测试，并采集和分析样本 作为Vet-LIRN调查的一部分。这项建议的目的是验证空肠弯曲菌Gyra 检测牛粪的聚合酶链式反应和用新的取代老化的实时聚合酶链式反应设备 与其他兽医诊断实验室中使用的设备兼容的设备，用于检测 用仪器从食源性病原体中提取核酸。对该矩阵方法的验证 将允许快速、有效地检测动物样本中的病原体，以支持FDA VET-LIRN通过迅速识别疫情来保护人类和动物健康的使命 以及对与疫情有关的样本进行检测。

PROJECT SUMMARY Foodborne illness causes significant morbidity and mortality each year in the United States. Salmonella, Campylobacter, and Listeria, all cause infections in people and animals. Although human foodborne illness is usually associated with the consumption of contaminated food, pets can be direct and indirect sources of bacterial pathogens. The Food and Drug Administration (FDA) Veterinary Laboratory Integrated Response Network (Vet-LIRN) Veterinary Diagnostic Laboratory Cooperative Agreement Program is designed to promote human and animal health by providing scientific information and building laboratory capacity for routine and emergency response for investigation of outbreaks and to investigate problems with animal feeds and drugs. Detection of outbreaks requires the ability to collect samples from geographically diverse areas. Texas is home to 25 million people, second only to California in human population, and leads the nation in horse and cattle populations. It is therefore important to understand the dynamics of foodborne diseases among animals and people within the State of Texas. The Texas A&M University Veterinary Medical Teaching Hospital Clinical Microbiology Laboratory currently serves as a FDA Vet-LIRN Laboratory. Members of the laboratory have helped to develop Vet-LIRN methods for detection, including molecular detection, of Salmonella and Campylobacter jejuni, participated in proficiency testing, and collecting and analyzing samples as part of Vet-LIRN investigations. The objective of this proposal is to validate the C. jejuni gyrA PCR for testing bovine feces and to replace aging real-time PCR equipment with new equipment compatible with that used in other veterinary diagnostic laboratories for detection of nucleic acid from foodborne pathogens with equipment. Validation of the method for this matrix will allow rapid, efficient detection of pathogens in samples from animals to support the FDA Vet-LIRN mission of protecting human and animal health by prompt recognition of outbreaks and testing of samples associated with outbreaks.