Core B: Transgenic and Gene-Targeting Institutional Facility

项目总结/摘要 使用基因改变的小鼠和干细胞模型是COBRE研究成员的关键工具 堪萨斯医科大学细胞发育和分化的分子调控项目 中心这些模型的产生和分析最终会导致对基本机制的更好理解 分化和基因表达，疾病进展的功能基因组学，以及 诊断和治疗。转基因和基因靶向共享资源（TGIF），由杰伊L。薇薇安 博士，支持COBRE成员和研究人员在堪萨斯城地区提供集中的技术 为生产新型转基因和基因靶向啮齿动物以及基因改变的多能 干细胞该设施使用最先进的方法，最先进的仪器和新型试剂， 这些模型的生成。TGIF的COBRE支持允许制定具体举措 与这些研究人员有关的设施。最近的举措包括成功地将新兴的 技术，包括体内基因组编辑方法（例如CRISPR/Cas9和TALE核酸酶），以提供一种新的基因组编辑方法。 更快速的方法来产生新的小鼠模型。这些不断发展的方法的整合 TGIF的“工具箱”大大加速了动物和干细胞模型的发展 和差异化，同时也降低了COBRE研究人员的总体成本。

Project Summary / Abstract The use of genetically altered mouse and stem cell models are critical tools for research members of the COBRE Project in Molecular Regulation of Cell Development and Differentiation at the University of Kansas Medical Center. The production and analysis of such models ultimately lead to better understanding of basic mechanisms of differentiation and gene expression, functional genomics of disease progression, and in vivo models for diagnostics and treatment. The Transgenic and Gene Targeting shared resource (TGIF), led by Jay L. Vivian PhD, supports members of COBRE and researchers in the Kansas City area by providing centralized technical services for the production of novel transgenic and gene-targeted rodents and genetically altered pluripotent stem cells. The Facility uses cutting edge methods, state-of-the-art instrumentation, and novel reagents for the generation of these models. The COBRE support of the TGIF allows for the development of specific initiatives in the Facility relevant to these researchers. Recent initiatives include successful integration of emerging technologies, including in vivo genome editing methods (e.g. CRISPR/Cas9 and TALE nucleases) to provide a more rapid means of generating novel mouse models. The integration of these continually evolving methods into the `toolbox' of the TGIF greatly accelerates the development of animal and stem cell models of development and differentiation, while also reducing the overall costs to COBRE researchers.