Transgenic & Gene-Targeting Shared Resource

转基因与基因打靶共享资源(TGTSR) 转基因模型是堪萨斯大学癌症中心研究人员的重要工具 (KUCC)。使用基于CRISPR的基因组编辑方法制作和分析此类模型，包括 小鼠、多能干细胞和肿瘤细胞系模型，最终导致更好地理解 肿瘤形成的本质、进展和功能基因组学。基因工程小鼠品系也 作为诊断和治疗的活体模型。用来产生基因的技术 改良的动物和细胞模型需要专门的设备和技术专长。转基因和 由Jay L.Viyan博士领导的基因打靶共享资源(TGTSR)通过以下方式支持KUCC成员 为新型基因工程产品的生产提供集中、全面的技术服务 啮齿动物和细胞系。TGTSR使用尖端方法、最先进的仪器和新颖的 用于产生这些模型的试剂。基因组编辑方法是该组织活动的核心 TGTSR，包括在胚胎和细胞模型中设计和优化使用CRISPR试剂的管道。 TGTSR主任和工作人员的专业知识在基因创新的所有阶段都得到了利用 工程模型，从最初的实验设计阶段到模型生成，分子 鉴定、扩增、基因分型和冷冻保存。这一被广泛使用的共享资源 在2020财年支持28个KUCC用户，并在TGTSR中支持49个唯一的KUCC成员 上一个供资期间，这表明了这一共享资源的广泛使用。五名全职工作人员 包括堪萨斯大学医学中心工作人员之间的协调努力 (KUMC)和儿童慈悲堪萨斯城(CM)校园，允许扩大工作人员以应对 KUCC成员的使用量增加。通过在KUCC财团之间集中操作和试剂 会员们，所有校区的KUCC调查人员都可以获得所有服务，大大减少了时间和成本。 癌症中心对TGTSR的支持允许制定与癌症相关的具体倡议 研究。例如，某些转基因方法和突变与癌症研究特别相关， 包括组织特异的转基因表达和微妙的突变，概括了临床上已确定的 变异和体细胞突变。KUCC的战略投资使其能够收购新的 在受精卵和细胞系中开发新的CRISPR突变方法的仪器和支持。 将这些不断发展的方法集成到TGTSR的“工具箱”中，极大地加速了 开发癌症的动物和细胞模型，同时降低KUCC研究人员在所有方面的成本 校园。

TRANSGENIC AND GENE-TARGETING SHARED RESOURCE (TGTSR): ABSTRACT Genetically altered models are important tools for the researchers at The University of Kansas Cancer Center (KUCC). The production and analysis of such models using CRISPR-based genome editing methods, including mouse, pluripotent stem cell, and tumor cell line models, ultimately leads to a better understanding of the nature, progression, and functional genomics of tumor formation. Genetically engineered mouse strains also serve as in vivo models for diagnostics and treatment. The techniques employed to generate genetically modified animal and cell models require specialized equipment and technical expertise. The Transgenic and Gene-Targeting Shared Resource (TGTSR) led by Jay L. Vivian, PhD, supports members of KUCC by providing centralized and comprehensive technical services for the production of novel genetically engineered rodents and cell lines. The TGTSR uses cutting-edge methods, state-of-the-art instrumentation, and novel reagents for the generation of these models. Genome editing methods are central to the activities of the TGTSR, including a pipeline for the design and optimized use of CRISPR reagents in embryo and cell models. The expertise of the TGTSR Director and staff is leveraged in all phases of the generation of novel genetically engineered models, from the initial experimental design stage through model generation, molecular characterization, expansion, genotyping, and cryopreservation. This extensively used shared resource supported 28 KUCC users in CY2020, and 49 unique KUCC members were supported by the TGTSR in the previous funding period, demonstrating the broad use of this shared resource. The five full-time staff members of the TGTSR include the coordinated efforts between staff at both the University of Kansas Medical Center (KUMC) and Children’s Mercy Kansas City (CM) campuses, allowing for staff expansion in response to increased use by KUCC members. By centralizing operations and reagents between KUCC consortium members, all services are available to KUCC investigators on all campuses at a greatly reduced time and cost. The Cancer Center support of the TGTSR allows for the development of specific initiatives relevant to cancer research. For example, certain transgenic methods and mutations are particularly relevant to cancer studies, including tissue specific transgene expression and subtle mutations that recapitulate clinically identified variants and somatic mutations. Strategic investments by the KUCC have allowed for acquisition of new instrumentation and support to develop novel CRISPR mutagenesis methods in the zygote and in cell lines. The integration of these continually evolving methods into the ‘toolbox’ of the TGTSR greatly accelerates the development of animal and cell models of cancer, while also reducing costs to KUCC researchers on all campuses.