Transgenic & Gene-Targeting Shared Resource
转基因
基本信息
- 批准号:9975737
- 负责人:
- 金额:$ 9.64万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-07-11 至 2022-06-30
- 项目状态:已结题
- 来源:
- 关键词:Animal Cancer ModelAnimal HusbandryAnimal ModelAnimalsBasic ScienceBiopsyCRISPR/Cas technologyCancer CenterCancer Center Support GrantCell LineClinicalClinical ResearchComplexConsumptionDataDetectionDevelopmentDiagnosticDoctor of PhilosophyEducational workshopEmbryoEmerging TechnologiesEquipmentEtiologyExperimental DesignsGelGene TargetingGene Transfer TechniquesGene-ModifiedGenerationsGeneticGenetically Modified AnimalsGenotypeHumanInjectionsIntegraseKansasMaintenanceMalignant NeoplasmsMethodologyMethodsMicroinjectionsMitochondriaModelingModificationMonitorMouse StrainsMusMutationNatureNuclearNucleic AcidsPatientsPhysiologicalPluripotent Stem CellsProcessProductionRattusReagentResearchResearch PersonnelResource SharingRodentRoleSamplingServicesSiteSomatic MutationStudentsTechnical ExpertiseTechniquesTechnologyTherapeuticTimeTissuesTrainingTransgenic OrganismsUniversitiesValidationVariantWorkanimal model developmentanticancer researchbaseblastocystcostdesignembryo cryopreservationembryonic stem cellexperimental studyfunctional genomicsgenetic manipulationgenome editinggenomic datahuman pluripotent stem cellin vivoin vivo Modelinduced pluripotent stem cellinstrumentationlecturesmembermouse geneticsmouse modelnew technologynovelnovel therapeuticsnuclear transferoperationresponsesperm cryopreservationstemstem cell biologystem cell modeltooltranscription activator-like effector nucleasestransgene expressiontumortumor progressiontumorigenesis
项目摘要
Transgenic and Gene-Targeting Shared Resource – Project Summary
Genetically altered mouse models are important tools for the researchers at the University of Kansas Cancer
Center (KUCC). The production and analysis of such models ultimately leads to a better understanding of the
nature, progression and functional genomics of tumor formation. They also serve as in vivo models for
diagnostics and treatment. The Transgenic and Gene-Targeting shared resource (TGTSR), led Jay L. Vivian
PhD, supports members of KUCC by providing centralized and comprehensive technical services for the
production of novel transgenic and gene-targeted rodents and genetically altered pluripotent stem cells. The
TGTSR uses cutting edge methods, state-of-the-art instrumentation, and novel reagents for the generation of
these models. The TGTSR, housed at the KUMC campus, has four full time technical staff along with Vivian.
The Cancer Center support of the TGTSR allows for the development of specific initiatives in the Facility
relevant to cancer research. For example, certain transgenic methods and mutations are particularly relevant
to cancer studies, including tissue specific transgene expression, subtle mutations that recapitulate clinically
identified variants and somatic mutations and strain-specific nuclear transfer. Emerging technologies, including
in vivo genome editing methods (e.g. CRISPR/Cas9 and TALE nucleases) are providing a more rapid means
of generating these types of novel mouse models of tumor progression. The integration of these continually
evolving methods into the `toolbox' of the TGTSR greatly accelerates the development of animal models of
cancer, while also reducing costs to KUCC researchers on all campuses.
转基因和基因靶向共享资源-项目摘要
对于堪萨斯癌症大学的研究人员来说,基因改变的小鼠模型是重要的工具
中心(KUCC)。这些模型的制作和分析最终会使人们更好地理解
肿瘤形成的性质、进展和功能基因组学。它们也可作为体内模型,
诊断和治疗。转基因和基因靶向共享资源(TGTSR),领导杰伊L。薇薇安
博士,支持KUCC的成员提供集中和全面的技术服务,
生产新型转基因和基因靶向啮齿动物和遗传改变的多能干细胞。的
TGTSR使用尖端的方法,最先进的仪器和新型试剂来产生
这些模型。TGTSR位于KUMC校园内,有四名全职技术人员沿着。
TGTSR的癌症中心支持允许在该设施中制定具体举措
与癌症研究有关。例如,某些转基因方法和突变特别相关,
癌症研究,包括组织特异性转基因表达,临床上重现的细微突变,
鉴定的变体和体细胞突变以及菌株特异性核转移。新兴技术,包括
体内基因组编辑方法(例如CRISPR/Cas9和TALE核酸酶)提供了更快速的手段,
产生这些类型的肿瘤进展的新型小鼠模型。这些不断的整合
将方法演变成TGTSR的“工具箱”,大大加速了动物模型的发展。
癌症,同时也降低了所有校园的KUCC研究人员的成本。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JAY L VIVIAN其他文献
JAY L VIVIAN的其他文献
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{{ truncateString('JAY L VIVIAN', 18)}}的其他基金
Core B: Transgenic and Gene-Targeting Institutional Facility
核心 B:转基因和基因靶向机构设施
- 批准号:
10215556 - 财政年份:2017
- 资助金额:
$ 9.64万 - 项目类别:
KANSAS U COBRE: GENETIC MODELS OF CONGENITAL VASCULAR MALFORMATIONS
堪萨斯大学 COBRE:先天性血管畸形的遗传模型
- 批准号:
8167982 - 财政年份:2010
- 资助金额:
$ 9.64万 - 项目类别:
KANSAS U COBRE: GENETIC MODELS OF CONGENITAL VASCULAR MALFORMATIONS
堪萨斯大学 COBRE:先天性血管畸形的遗传模型
- 批准号:
7959575 - 财政年份:2009
- 资助金额:
$ 9.64万 - 项目类别:
KANSAS U COBRE: GENETIC MODELS OF CONGENITAL VASCULAR MALFORMATIONS
堪萨斯大学 COBRE:先天性血管畸形的遗传模型
- 批准号:
7721037 - 财政年份:2008
- 资助金额:
$ 9.64万 - 项目类别:
PHENOTYPIC SCREENS OF THE TGF-BETA TUMOR SUPPRESSOR PATHWAY IN MOUSE ES CELLS
小鼠 ES 细胞中 TGF-β 肿瘤抑制途径的表型筛选
- 批准号:
7609713 - 财政年份:2007
- 资助金额:
$ 9.64万 - 项目类别:
KANSAS U COBRE: GENETIC MODELS OF CONGENITAL VASCULAR MALFORMATIONS
堪萨斯大学 COBRE:先天性血管畸形的遗传模型
- 批准号:
7610807 - 财政年份:2007
- 资助金额:
$ 9.64万 - 项目类别:
PHENOTYPIC SCREENS OF THE TGF-BETA TUMOR SUPPRESSOR PATHWAY IN MOUSE ES CELLS
小鼠 ES 细胞中 TGF-β 肿瘤抑制途径的表型筛选
- 批准号:
7381092 - 财政年份:2006
- 资助金额:
$ 9.64万 - 项目类别:
Phenotypic screen in mouse embryonic for TGF-beta signaling mutations
小鼠胚胎中 TGF-β 信号突变的表型筛选
- 批准号:
7082457 - 财政年份:2006
- 资助金额:
$ 9.64万 - 项目类别:
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