Mouse Core

这个核心实验室的总体目标是提供基础设施、材料、动物、技术专长 这将有助于在检测HIV的研究中使用人源化免疫缺陷小鼠 体内储库。最近的进展使得使用缺乏自身免疫系统的小鼠， 植入人体组织将能够发育成人类免疫细胞的人类组织置于 在小鼠体内，它会发育成多种类型的人类免疫细胞。使用的人体组织 在这些研究中，能够在小鼠和人类中进行高度的操作和实验， 与小鼠细胞相比，这些细胞更容易感染HIV。这提供了一个强大的模型， 检查人类血细胞的发育，研究艾滋病毒感染对人类细胞的影响，并检查 从免疫细胞中清除艾滋病毒的方法。这些人源化小鼠的产生是一种高度专业化的 程序，由于需要免疫缺陷小鼠品系、人造血组织、感染性 材料、专用设施以及在该系统中进行实验所需的技能和知识。 这个新的核心将代表一个独特的和宝贵的资源，为这个计划，作为主任的核心 拥有超过24年的人性化小鼠模型的经验，核心团队的工作人员帮助 在这些模型中开创了各种新颖的技术和发现。为了使我们能够准确地评估和 比较我们的结果，小鼠模型的标准化变得绝对关键。因此，对于 这个程序，为了得出正确的结论，涉及人源化小鼠的研究， 实验室之间的标准化。该中心的主要目的是提供专门服务， 本计划中所有3个项目所需的标准化资源， 一致和可靠的数据。

The overall goal of this Core laboratory is to provide the infrastructure, materials, animals, technical expertise and support that will facilitate the use of humanized immunodeficient mice in studies that examine HIV reservoirs in vivo. Recent advances have enabled the use of mice that lack their own immune system mice to be implanted with human tissue. Human tissue capable of developing into human immune cells is placed in the mice where it undergoes development into multiple types of human immune cells. The human tissue used in these studies is capable of a high degree of manipulation and experimentation in the mouse and the human cells, versus the mouse cells, are further susceptible to infection with HIV. This provides a powerful model to examine human blood cell development, to study the effects of HIV infection on human cells, and examine ways to purge HIV from immune cells. The generation of these humanized mice is a highly specialized procedure, due to the requirement for immunodeficient mouse strains, human hematopoietic tissue, infectious material, specialized facilities, and the necessary skill and knowledge to perform experiments in this system. This new core would represent a unique and valuable resource for this program, as the director of the Core has greater than 24 years of experience with humanized mouse models and the staff of the core have helped pioneer various novel techniques and findings in these models. In order for us to accurately assess and compare our results, standardization of the mouse model becomes absolutely critical. Thus, it is essential for this program, in order to draw the proper conclusions, that the studies involving humanized mice be standardized between laboratories. The central purpose of this core is to provide the specialized services and resources needed by all 3 projects in this program in the standardized fashion necessary to generate consistent and reliable data.