Proteomics & Metabolomics Core

摘要-蛋白质组学和代谢组学共享资源 DCI蛋白质组和代谢组学共享资源(DPM)提供了种类繁多的质量 基于光谱的非靶向(组学)和靶向定性和定量能力，用于蛋白质组学和 用于支持基础科学研究所需的靶向代谢组学。该实验室也有所需的规模 支持临床研究，包括生物标记物发现和生物标记物验证实验。 DPM配备了七个高分辨率质谱仪，三个三重四极杆质量 光谱仪和11个超性能液相色谱仪(UPLC)系统。该设备包括 在过去18个月内购买了四台最先进的质谱仪-Fusion Lumos配有 电子转移解离(THERMO)、电子转移解离与紫外光的熔合流明 光解离(Thermo)、Q Exactive HF-X(Thermo)和TQ-XS(Waters)。DPM有全职工作人员 11名科学家，包括6名博士(4名研究教授、1名科学工作人员和1名博士后) DOC)。这个团队在质谱学方面有超过195年的综合经验，并撰写了379多篇 或合著出版物(将两个DMP成员合著的出版物算作两个作者- 出版物)。该资源位于一个新的(2017)~8,100平方米。英国《金融时报》切斯特菲尔德大楼的实验室 达勒姆市中心，为基于质谱学的蛋白质组学和新陈代谢组学定制。通过密切合作 随着供应商获得广泛的折扣，审慎使用DPM改善资金用于新设备， 和DCI CCSG支持，DPM保持了对DCI的经济高效(收入中性)支持， 最先进的水平。DPM的目标是通过向其提供全面服务来生成高质量的数据 顾客。这包括初步咨询以创建最佳实验设计，使用严格的QC 从样品制备到样品分析和生成详细报告的方案和标准 确保实验方法和结果的清晰度。实验室信息管理系统的使用 (LIMS)确保样品的完整性(由自动分配的唯一项目编号和样品IS编号 LIMS)，并且使用真正的“指纹”数据存储库(“GCB Express数据存储库”)来确保数据 正直。 2018年，DPM为95个DCI成员(41%的独立用户)提供了服务，他们占17% 总使用量，代表了8个DCI研究项目中的7个。DCI成员对此共享资源的使用 在项目期间发表了65份出版物，其中19份刊登在影响较大的期刊上(如果&>9)， 展示资源提供的服务的价值。在此期间，DPM得到了以下方面的支持 DCI CCSG和SOM以新实验室成本的形式提供的机构支持(设计和建造新的 (切斯特菲尔德大楼的设施)和新的仪器(三台最先进的质谱仪)。 R：\2019竞争更新\共享资源\蛋白质组学和代谢组学\最终文档\P&M 摘要最终评审1.14.19.docx

ABSTRACT – PROTEOMICS AND METABOLOMICS SHARED RESOURCE The DCI Proteomics and Metabolomics Shared Resource (DPM) provides the wide variety of mass spectrometry-based untargeted (‘omic) and targeted qualitative and quantitative capabilities for proteomics and for targeted metabolomics which are needed to support basic science studies. The lab also has the scale needed for support of clinical research studies, including biomarker discovery and biomarker verification experiments. The DPM is equipped with seven high-resolution mass spectrometers, three triple-quadrupole mass spectrometers, and eleven Ultra Performance Liquid Chromatography (UPLC) systems. This equipment includes four state-of-the-art mass spectrometers purchased within the past 18 months – a Fusion Lumos Tribrid with Electron Transform Dissociation (Thermo), Fusion Lumos Tribrid with Electron Transform Dissociation and UV Photodissociation (Thermo), a Q Exactive HF-X (Thermo) and a TQ-XS (Waters). The DPM has a full-time staff of eleven scientists, including six PhDs (four Research Professors, one scientific staff member and one Post- Doc). This group has over 195 years’ combined experience in mass spectrometry and more than 379 authored or co-authored publications (counting publications with two DMP members as co-authors as two author- publications). The resource is located in a new (2017) ~8,100 sq. ft. laboratory in the Chesterfield Building in downtown Durham, custom built for mass spectrometry-based proteomics and metabolomics. By working closely with vendors to obtain extensive discounts, prudent use of DPM Betterments Funds towards the new equipment, and the DCI CCSG support, the DPM has maintained cost-effective (revenue neutral) support for the DCI at state-of-the-art levels. The DPM’s objective is to generate high-quality data by providing full service to its customers. This includes initial consultations to create optimal experimental designs, use of rigorous QC protocols and standards from sample preparation through sample analyses, and generation of detailed reports to ensure clarity on experimental approach and results. Use of a Laboratory Information Management System (LIMS) ensures sample integrity (unique Project Numbers and Sample IS numbers automatically assigned by the LIMS), and the use of a true ‘fingerprinted’ data repository (“GCB Express Data Repository”) ensures data integrity. In 2018, the DPM provided services to 95 DCI members (41% of unique users), who accounted for 17% of total usage and represented 7 of the 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 65 publications over the project period, 19 of which were in high impact journals (IF>9), demonstrating the value of services offered by the resource. During this time, the DPM received support from the DCI CCSG and Institutional Support from the SoM in the form of new lab costs (design and build of new facility in the Chesterfield building) and new instrumentation (three state-of-the-art mass spectrometers). R:\2019 Competing Renewal\Shared Resources\Proteomics and Metabolomics\FINAL Documents\P&M Abstract Final Review 1.14.19.docx