Translational Biomarker Analytical Core (TBAC)

转化型生物标志物分析核心(TBAC)实验室向DRC提供 研究人员使用分析技术支持糖尿病、胰岛素抵抗、 糖尿病在人类受试者和动物中的中间代谢和医学后果 模特们。所提供的服务最好设在核心实验室，因为它们需要调查人员 难以或不切实际的咨询、特殊手段和方法 单独的实验室。此外，这种安排允许协调注意可靠性和 所用分析方法的重现性。在研究葡萄糖动态平衡，胰岛素分泌， 血脂异常、激素作用、分子遗传学或药物效应，都必须能够监测 胰岛素、C肽、胰高血糖素、GLP-1、GIP、PYY、皮质醇和儿茶酚胺等激素以及 葡萄糖、游离脂肪酸、甘油、酮、氨基酸和类脂等代谢物。随着不断增加的 开发和验证新的疾病生物标记物的技术能力，这是 核心是尽快开发和创新这些新方法，以便研究基地能够 利用这些化验结果。在过去的筹资周期中，核心中心向33个刚果民主共和国提供了约80,000项化验 获得81笔赠款支持的成员。由此产生的数据已经为NIH提供了17笔新的拨款， 85份核心支助出版物(69份为主要出版物，16份为次要出版物)。具体目标 TBAC的特点是：1)为了满足研究基地对动态范围生物检测的高要求， 提高效率、质量和重复性，同时最大限度地降低成本。2)回应议员的意见 通过引入利用核心工作人员的专业知识的新的分析和方法来满足需求。3)至 为研究人员及其同事提供分析方法、实验设计、 和数据解释。4)培训初级调查人员和博士后研究员在 核心方法，并在适当的情况下将分析方法转移到各自的实验室。5)方便使用 由哥伦比亚CTSA生物标记物实验室进行靶向代谢组学/脂质组学测定。6) 为了方便接触生物信息学、动力学研究和途径分析方面的专家， 正确解释代谢组学/脂质组学测定和稳定同位素动力学研究。7) 促进研究基地之间的合作，宣传可用的资源，并开发 和/或促进调查人员获得新技术，例如基于MS的方法来分析和 量化代谢物。TBAC的分析和咨询能力与 其他DRC核心提供定量代谢分析的综合方法(图1)。

The Translational Biomarker Analytical Core (TBAC) Laboratory makes available to DRC investigators analytical techniques to support studies of diabetes mellitus, insulin resistance, intermediary metabolism, and medical consequences of diabetes in human subjects and animal models. The services provided are best based in a Core Laboratory because they require investigator consultation, special instrumentation and methods that are difficult or impracticable to establish in individual laboratories. In addition, this arrangement allows for coordinated attention to reliability and reproducibility of the assays employed. In investigations of glucose homeostasis, insulin secretion, dyslipidemia, hormone action, molecular genetics, or drug effects, it is essential to be able to monitor hormones such as insulin, C-peptide, glucagon, GLP-1, GIP, PYY, cortisol and catecholamines and such metabolites as glucose, FFA, glycerol, ketones, amino acids, and lipids. With the increasing technical capabilities to develop and validate novel disease biomarkers, one of the key functions of the Core is to expeditiously develop and inaugurate these new methods so that the research base can avail itself of these assays. In the past funding cycle, the Core provided ~80,000 assays to 33 DRC members supported by 81 grants. The resultant data have contributed to 17 new NIH grants, and 85 Core-supported publications (69 as primary, 16 as secondary Core). The SPECIFIC AIMS of the TBAC are: 1) To meet the high demand for a dynamic range of bioassays by the research base, enhancing efficiency, quality, and reproducibility, while minimizing costs. 2) To respond to members’ needs by introducing new assays and methods that leverage the expertise of the Core staff. 3) To provide expert advice to investigators and their associates on analytical methods, experimental design, and data interpretation. 4) To train junior investigators and post-doctoral fellows in practical skills in the Core methods and, if appropriate, transfer analytical methods to their own labs. 5) To facilitate access to targeted metabolomics/lipidomics determinations by the Columbia CTSA Biomarker Laboratory. 6) To facilitate access to experts in bioinformatics, kinetic studies and pathway analyses required to appropriately interpret metabolomics/lipidomics determinations and stable isotopes kinetic studies. 7) To facilitate collaborations among the research base, advertise available resources, and develop and/or facilitate investigator access to new technologies, such as MS-based methods to analyze and quantify metabolites. TBAC analytic and consultative capabilities are closely articulated with the other DRC Cores to provide an integrated approach to quantitative metabolic analyses (Fig. 1).