Core B: Proteomics and Metabolomics

核心B摘要 蛋白质组学和代谢组学核心(核心B)提供最先进的仪器、方法和 在这个PPG的研究人员在分析蛋白质组学和代谢组学方面的专业知识。的一项独特功能 蛋白质组学和代谢组学的核心是前沿定量蛋白质组学分析的可用性 允许对胃癌模型中蛋白质组变化进行高灵敏度测量的技术。新的 这一更新应用中的服务包括发现和靶向代谢组学以及组织成像 质谱仪(IMS)。核心提供1)复杂样品中蛋白质的鉴定和定量， 2)蛋白质修饰分析，包括磷酸化；3)代谢物定量分析 4)利用IMS对胃组织进行分子成像。 核心工作人员提供实验设计咨询以及亲手样品制备、质量 光谱分析和原始数据分析。用于LC的多台高性能质谱仪 MS/MS实验可用于建议的分析。此外，还提供了先进的方法 包括：用于从复杂样品中鉴定蛋白质的多维LC-MS/MS，TagRecon数据库 用于从MS/MS数据中识别意外修饰或序列变体的搜索分析，以及 用于组织分析的高空间和质量分辨率成像方法。核心还提供了相关的 使用稳定同位素差异标记策略iTRAQ和SILAC对来自以下来源的样品进行定量 动物模型或细胞培养。蛋白质表达或代谢物丰度的验证 改变是使用有针对性的多反应监测LC-MS/MS方法来完成的。 项目1(PEEK)将利用代谢组学方法来测量分离的幽门螺杆菌菌株的代谢物变化。 从受感染的铁耗尽或补充沙土鼠作为时间的函数。项目2(威尔逊)将利用磷化硅胶 为了测量磷蛋白质组和代谢组学的变化，以测量代谢产物的变化 巨噬细胞对幽门螺杆菌感染和EGFR或DHPS的抑制作用。项目3(封面)将雇用 代谢组学方法检测血浆和胃组织中代谢物的变化。此外，这个项目 将利用IMS方法测量胃组织中代谢物、蛋白质和脂质的变化- 解决的方式作为饮食盐，感染和炎症的功能。 好了！

Core B Summary The Proteomics and Metabolomics Core (Core B) provides state-of-the-art instrumentation, methodology, and expertise in analytical proteomics and metabolomics to investigators in this PPG. A unique feature of the Proteomics and Metabolomics Core is the availability of cutting-edge quantitative proteomics analysis technologies that allows high sensitivity measurements of proteome changes in models of gastric cancer. New services in this renewal application include discovery and targeted metabolomics as well as tissue imaging mass spectrometry (IMS). The Core provides 1) identification and quantitation of proteins in complex samples, 2) analyses of protein modifications, including phosphorylation, 3) quantitative analyses of metabolite abundances, and 4) molecular imaging of gastric tissue using IMS. Core staff provide consultation on experimental design as well as hands on sample preparation, mass spectrometry analysis and primary data analysis. Multiple high performance mass spectrometers for LC- MS/MS experiments are available for proposed analyses. In addition, advanced methods are available including: multidimensional LC-MS/MS for protein identification from complex samples, TagRecon database searching analysis for identification of unanticipated modifications or sequence variants from MS/MS data, and high spatial and mass resolution imaging methods for tissue analysis. The Core also provides relative quantitation using stable isotope differential labeling strategies, iTRAQ and SILAC, for samples derived from animal models or from cell culture, respectively. Validation of protein expression or metabolite abundance changes is accomplished using targeted multiple reaction monitoring LC-MS/MS methods. Project 1 (Peek) will utilize metabolomics methods to measure metabolite changes in H. pylori strains isolated from infected iron deplete or replete gerbils as a function of time. Project 2 (Wilson) will utilize phospho-SILAC to measure changes in the phosphoproteome and metabolomics to measure metabolite changes in macrophages upon H. pylori infection and with EGFR or DHPS inhibition. Project 3 (Cover) will employ metabolomics methods to examine metabolite changes in plasma and gastric tissue. In addition, this project will utilize IMS methods to measure changes in metabolites, proteins and lipids in gastric tissue in a spatially- resolved manner as a function of dietary salt, infection and inflammation. !