Deposition of GBM invasion chip data to the Microphysiology Systems Database

项目总结 摘要胶质母细胞瘤是最常见和最具侵袭性的原发恶性脑肿瘤。 肿瘤。由于其趋化性，患者的中位生存期只有15个月。 通过3D-受限的间质空间侵入邻近脑组织。这个 其发病机制尚不清楚，缺乏有效的治疗方法。 这是由于缺乏足够的研究平台。目前没有 这种疾病在微生理学系统数据库中的表现。 通过一项积极的NIH赠款研究，我们最近开发了一种微流控设备来研究 GBM入侵。这种设备概括了个体癌症的自然环境。 细胞在间质中迁移。我们进一步整合了化学灌流 可以操纵趋化环境的系统(例如，化学成分， 绝对浓度、浓度梯度)。到目前为止，我们已经收集了一个 大量的细胞图像和迁移数据，在各种趋化条件下。 我们还收集了一种潜在抑制药物的药代动力学数据。 为了向公众提供这些数据，我们建议将它们重新格式化并存放在 微生理学系统数据库。我们还将通过收集 来自其他患者来源细胞系的药代动力学数据。这项研究的结果 将大大提高我们对GBM入侵的了解，并促进 发现更有效的治疗方法。

PROJECT SUMMARY Glioblastoma (GBM) is the most prevalent and aggressive primary malignant brain tumor. The patient median survival is only fifteen months, due to its chemotactic invasion into adjacent brain tissues through the 3D-confined interstitial space. The underlying mechanism is still poorly understood, and effective therapies are still lacking. This is due to the lack of adequate research platforms. There is currently no representation of this disease in the Microphysiology System Database. Through an active NIH grant study, we recently developed a microfluidic device to study GBM invasion. This device recapitulates the native environment for individual cancer cell migration through interstitial space. We further integrated the chemical perfusion system that can manipulate the chemotactic environment (e.g., chemical composition, absolute concentration, concentration gradient) in real-time. So far, we have collected a significant amount of cell images and migration data, in various chemotactic conditions. We also collected the pharmacokinetic data for a potential inhibitory drug. To render the data to the general public, we propose to re-format and deposit them in the Microphysiology System Database. We will also expand the dataset by collecting the pharmacokinetic data from additional patient-derived cell lines. This study's outcome will significantly enhance our understanding of the GBM invasion and promote the discovery of more effective therapy.