WaNPRC Macaca nemestrina SPF Breeding Colony

项目概要/摘要： 本计画的目的是维持及提升无特定病原体（SPF）猪尾猕猴（M。 nemestrina）繁殖群在华盛顿国家灵长类动物研究中心（WaNPRC）。这个殖民地是 国内最大的M. nemestrina和这种重要动物模型的主要来源 艾滋病研究和其他类型的生物医学研究在美国。M. nemestrina有独特的 免疫学、遗传学、行为学、解剖学和生理学特征，使它们成为一种必需的 在一些与艾滋病毒/艾滋病有关的研究领域，特异性病原体（SIV、SRV、STLV-1、McHV-1） 对动物或人类健康构成威胁或干扰研究，必须排除以优化非人类 灵长类研究模型在本提案中，我们讨论了如何维护WaNPRC SPF M。nemestrina 育种群体提供最高质量的动物，以满足研究需求。我们正在 在我们亚利桑那州的育种设施巩固育种业务。SPF的饲养和维护 繁殖群体，包括财务可持续性，在核心1、畜牧和管理核心中进行了描述。 为确保殖民地保持SPF而进行的病毒检测见核心2，病毒检测 核心遗传管理和基因分型动物的亲子关系和主要组织相容性复合体 等位基因描述于核心3，MHC遗传分型核心。SPF M. Nemestrina殖民地作为一个 国家资源，为全国各地的艾滋病研究人员提供动物，优先考虑NIH资助的 investigators.

Project Summary/Abstract: The goal of this project is to maintain and enhance the specific pathogen free (SPF) pigtail macaque (M. nemestrina) breeding colony at the Washington National Primate Research Center (WaNPRC). This colony is the largest domestic breeding colony of M. nemestrina and the primary source of this important animal model for AIDS studies and other types of biomedical research in the United States. M. nemestrina have unique immunological, genetic, behavioral, anatomical, and physiological characteristics that make them an essential model in a number of areas of research related to HIV/AIDS. Specific pathogens (SIV, SRV, STLV-1, McHV-1) are a threat to animal or human health or interfere with research and must be excluded to optimize nonhuman primate research models. In this proposal, we discuss how we will maintain the WaNPRC SPF M. nemestrina breeding colony to provide animals of the highest quality to meet research needs. We are in the process of consolidating breeding operations at our Arizona breeding facility. Breeding and maintenance of the SPF breeding colony, including financial sustainability, are described in Core 1, Husbandry and Management Core. The viral testing that is performed to ensure that the colony remains SPF is described in Core 2, Viral Testing Core. Genetic management and genotyping animals for parentage and major histocompatibility complex alleles are described in Core 3, MHC Genetic Typing Core. The SPF M. nemestrina colony serves as a national resource, providing animals to AIDS researchers across the country, with priority given to NIH-funded investigators.