Orbitrap ID-X Tribrid Mass Spectrometer for Unbiased Global Metabolomics Profiling

摘要 该S10申请的目标是为高分辨率Orbitrap ID-X Tribrid质量申请资金。 光谱仪（OT IDX），能够识别和量化各种环境中的全球代谢物谱 生物标本这将填补贝勒医学院代谢组学核心中的“缺失环节”， date，擅长提供有针对性的代谢分析平台，以支持科学研究。OT IDX将 用于开发稳健且经过充分测试的方法，用于分析全球范围内代谢物的稳态水平 时尚以及全球范围内代谢通量的变化。这些技术将作为一种新的 代谢组学核心中的服务线将支持使用临床前标本（细胞系来源的 异种移植物、患者来源的异种移植物和转基因模型）、临床标本和细胞系。重要的是 无偏见的技术将支持癌症，糖尿病，肥胖，环境暴露， 病毒感染等。OT IDX将由Baylor代谢组学核心根据高级 技术核心保护伞。OT IDX的安装和实施将得到 机构资金将涵盖人员、服务合同、软件和培训成本。运作和 OT IDX的输出将由内部咨询委员会和外部咨询委员会监测， 有代谢组学专家，核心领导人和机构代表。根据未完成的 贝勒医学院代谢组学核心的跟踪记录，该核心是强大的定位，以建立 在机构中使用OT IDX的创新无偏见全球代谢平台。这将使调查人员能够 开发新的假设和线索，最终可能导致新的生物标志物和新的治疗干预措施。

ABSTRACT The goal of this S10 application is to request funding for a high resolution Orbitrap ID-X Tribrid mass spectrometer (OT IDX) to enable identification and quantification of global metabolite profiles in various biospecimens. This will fill a “missing link” in the Metabolomics Core at Baylor College of Medicine that has to date, excelled in offering targeted metabolic profiling platforms to support scientific investigations. OT IDX will be used to develop robust and well tested methods for profiling steady state levels of metabolites in a global fashion as well as changes in metabolic flux in a global scale. These technologies that will be offered as a new line of service in the Metabolomics Core will support studies using pre-clinical specimens (cell line-derived xenografts, patient-derived xenografts and transgenic models), clinical specimens and cell lines. Importantly, the unbiased technologies will support projects in the areas of Cancer, Diabetes, Obesity, Environmental Exposures, Viral infections, etc. The OT IDX will be administered by the Baylor Metabolomics Core under the Advanced Technology Core Umbrella. The installation, and implementation of OT IDX will be significantly supported by the institutional funding that will cover personnel, Service Contract, software and training costs. The functioning and output of the OT IDX will be monitored by the Internal Advisory Committee and External Advisory Committee, which have experts in metabolomics, Core leaders and institutional representatives. Based on the outstanding track record of the Metabolomics Core at Baylor College of Medicine, the Core is strongly positioned to establish the innovative unbiased global metabolic platform using OT IDX in the institution. This will allow investigators to develop novel hypothesis and leads that could culminate in new biomarkers and novel therapeutic interventions.