ACQUISITION OF THE YOKOGAWA CV8000 HIGH THROUGHPUT SPINNING DISK MICROSCOPE AND ROBOTICS

项目摘要/摘要 在这一应用中，贝勒医学院(德克萨斯州休斯敦BCM)的集成显微镜核心(IMC) 需要横河细胞旅行者8000(CV8000)高吞吐量旋转磁盘共焦平台。这个 CV8000还与机器人装板机和培养板的硬件/软件解决方案集成在一起 酒店允许全天候实时成像实验。这种类型的平台目前在休斯顿不能使用 或德克萨斯州的任何地方。IMC是一个长期的核心设施，部分由NCI资助的DAN提供支持 L邓肯综合癌症中心，NIDDK资助的德克萨斯医疗中心消化系统疾病中心 和CPRIT资助的墨西哥湾沿岸高级显微镜和图像信息学联盟中心 (Camii)。在过去的几年里，科学界越来越需要质疑 不同观察尺度的生物过程，在空间上(从单个细胞到有机体再到模型 生物体)，并在时间上(从几秒钟到几天)，并将发现转化为新的行动机制 和治疗途径。因为提高了分析开发和小型化的能力(96-384 井板)，现在可以在大量条件下同时查询模型系统(即， 小分子筛选)，并通过先进的图像分析对单个对象进行量化 多维空间(空间、时间、形状、纹理、强度)的表型/机械变化 高度复杂的、多参数的病理生理现象视图。 CV8000为我们提供了完成上述任务的独特可能性；它是一种状态- 最先进的行业标准，市场上无与伦比的高吞吐量旋转磁盘共焦，配有6个激光器 线，4个摄像头，专为实时和固定2D/3D的低(4倍)和高分辨率(60倍/水)成像而设计 样本。随附的Cell Pathfinder高内容图像分析软件将补充目前 可在IMC/CAMII上使用的生物信息学资源。孵化机器人平台的增加 PAA的平板加载器将允许我们执行短期和长期实时成像实验 核心人员的持续参与，或固定单元项目的潜在限制。 CV8000的多功能性和超高图像质量将为BCM和TMC的许多小组提供 结果和实验设计目前在现有资源下是不可能或不可行的。AS 提案中详细介绍了CV8000将为需要多维实时/固定成像的群体提供服务 有机物、模型生物和其他2D/3D细胞模型。与卡米亚和GCC合作放映 计划和贝勒药物发现中心，有许多小分子文库可供支持 种类繁多的筛选项目。CV8000将是IMC资源和电池的完美补充 将融入我们的教育使命(研讨会和研究生级别的显微镜课程)。

Project Summary/Abstract In this application, the Integrated Microscopy Core (IMC) at Baylor College of Medicine (BCM, Houston, TX) requests a Yokogawa Cell Voyager 8000 (CV8000) high throughput spinning disk confocal platform. The CV8000 also is integrated with a hardware/software solution for a robotic plate-loader and incubated plate hotel to allow 24/7 live imaging experiments. This type of platform is currently not available in the Houston area, or anywhere in Texas. The IMC is a long-standing core facility supported in part by the NCI-funded Dan L Duncan Comprehensive Cancer Center, the NIDDK-funded Texas Medical Center Digestive Disease Center and the CPRIT-funded Gulf Coast Consortium Center for Advanced Microscopy and Image Informatics (CAMII). In the last few years, there has been an increased need in the scientific community to query biological processes at different scales of observation, both spatially (from single cells to organoids to model organisms), and temporally (from seconds to days), and to translate findings into novel mechanisms of action and therapeutic avenues. Because of increased capabilities in assay development and miniaturization (96-384 well plates), it is now possible to simultaneously query model systems under a large number of conditions (i.e., small molecule screening), and, through advanced image analytics, to quantify single object phenotypic/mechanistic changes in multidimensional space (space, time, shape, texture, intensity) to create highly complex, multiparametric views of pathophysiological phenomena. The CV8000 provides us with the unique possibility of accomplishing the abovementioned tasks; it is a state- of-the-art, industry standard, unmatched on the market, high throughput spinning disk confocal, with 6 laser lines, 4 cameras, designed for low (4x) and high resolution (60x/water) imaging of live and fixed 2D/3D samples. The included Cell Pathfinder high content image analysis software will complement currently available bioinformatics resources available in the IMC/CAMII. The addition of the incubated robotic platform and plate-loader by PAA will allow us to perform short- and long-term live imaging experiments without continuous involvement of core personnel, or potential limitations from fixed-cell projects. The versatility and ultra-high image quality of the CV8000 will provide many groups at BCM and the TMC with results and experimental designs that are currently not possible or practical with available resources. As detailed in the proposal, the CV8000 will serve groups that need multidimensional live/fixed imaging of organoids, model organisms and other 2D/3D cell models. In partnership with CAMII and GCC Screening Program and the Baylor Center for Drug Discovery, numerous small molecule libraries are available to support a wide range of screening projects. The CV8000 will be a perfect addition to the battery of IMC resources and will be integrated in our educational mission (seminars and graduate level microscopy class).