High-Throughput DNA Sequencer

塔夫茨大学核心设施基因组学核心(TUGC)正在申请资金购买高通量 测序仪，Illumina NovaSeq 6000。这将是对基因组学核心的关键补充，以支持ITS 为Tuft内的研究社区提供高质量的下一代测序(NGS)的使命 大学及其附属机构。 TUGC成立于2008年，旨在满足对NGS的萌芽需求。由于PI的不懈努力 和他的同事们，它已经成长为塔夫茨大学和塔夫茨医学中心内唯一的核心设施 提供全面的NGS服务。核心以其知识渊博和随和的员工而闻名。 这将协作为NIH资助的调查人员提供端到端的NGS服务。除 作为标准样品制备和测序服务的一部分，TUGC向用户提供来自 通过数据分析和可视化进行初步的实验设计。核心一直在支持高- 涵盖各种健康和生命科学重点领域的影响研究，包括免疫学、微生物学和 宿主-病原体相互作用、细胞和癌症生物学、遗传和相关疾病、神经生物学和神经学 疾病、微生物组、发育与恢复生物学、环境与种群生物学、营养学 生物学和衰老。 多年来，基因组核心已经迭代了几代测序仪。目前，它提供 它的NGS服务包括几台Illumina仪器，包括一台HiSeq2500、一台NextSeq 550和两台MiSeq。 Illumina已经发布了停产公告，并将在2023年初停止对HiSeq仪器的支持。这个 停用将使其过时，并导致需要高数据收益率的项目出现严重的服务缺口 HiSeq提供的。因此，迫切需要替换定序器来防止中断 我们国家卫生研究院资助的研究人员正在进行的研究。确定HiSeq替代产品的最佳选择 需要考虑正在进行的和未来的研究项目的排序需求，当前的 仪器，TUGC的专业知识，设施的未来发展，以及新的应用。出于这些原因， Illumina NovaSeq 6000是最合理的选择。这是继HiSeq之后的最新型号 1000/2000/3000/4000系列，已成为高输出仪器仪表的首选。它支持 高达250个碱基的各种读取长度，并提供四种流动单元选择，以满足一系列数据产量 要求。24至48小时的输出能力从650亿到6万亿个基数和6.5亿个 400亿次读取，它提供了多个 用户环境。

The Tufts University Core Facility Genomics Core (TUGC) is applying for funds to purchase a high throughput sequencer, an Illumina NovaSeq 6000. It will be a critical addition to the Genomics Core in support of its mission to provide high-quality next-generation sequencing (NGS) for the research community within Tufts University and its affiliates. The TUGC was established in 2008 to address the budding need for NGS. Due to the tireless efforts of the PI and his colleagues, it has grown into the only core facility within Tufts University and Tufts Medical Center that offers a comprehensive NGS service. The core is renowned for its knowledgeable and accommodative staff that works collaboratively to provide end-to-end NGS services to NIH-funded investigators. In addition to the standard sample preparation and sequencing services, the TUGC offers its users advice and support from the initial experimental design through the data analysis and visualization. The core has been supporting high- impact research covering diverse health- and life-science focused areas including immunology, microbiology & host-pathogen interaction, cellular & cancer biology, genetic & associated diseases, neurobiology & neurologic diseases, microbiome, developmental & restorative biology, environmental & populational biology, nutritional biology, and aging. The Genomics Core has iterated through several generations of sequencers over the years. Currently, it offers its NGS services with several Illumina instruments including a HiSeq2500, a NextSeq 550, and two MiSeqs. Illumina has issued an end-of-life bulletin and will cease its support of the Hiseq instrument in early 2023. The end-of-life will render it obsolete and result in a severe service gap for projects that require the high data yield that the HiSeq provides. Thus, there is a dire need for a replacement sequencer to prevent an interruption to the ongoing research of our NIH-funded investigators. Determining the optimal choice of a HiSeq replacement requires considering the sequencing needs of ongoing and future research projects, the current array of instrumentation, TUGC expertise, the future growth of the facility, and new applications. For these reasons, the Illumina NovaSeq 6000 is the most logical choice. It is the latest model succeeding the HiSeq 1000/2000/3000/4000 series and has become the leading choice for high output instrumentation. It supports various read lengths of up to 250 bases and offers four choices of flow cells that cater to a range of data yield requirements. With a 24- to 48-hour output capacity ranging from 65 billion to 6 trillion bases and 650 million reads to 40 billion reads, it provides the flexibility, scalability, speed, and cost effectiveness required in a multi- user environment.