University of Michigan Skin Biology and Diseases Resource-based Center

项目概要-总体 密歇根大学皮肤生物学和疾病资源中心（UM-）的总体目标 SBDRC）是利用我们在基因组编辑，生物信息学和小鼠建模方面的优势， 从发现到功能的过渡，解决生物和疾病背景，并促进 适当的最先进的计算机模拟方法和疾病的体内小鼠模型。主要强调 UM-SBDRC将把多年的尖端小鼠建模技术应用于炎症皮肤的研究 利用通过大数据方法获得的新知识来研究疾病。因此，UM-SBDRC将有助于 并加速正在进行的研究，以实现新的发现，并最终受益于广泛的患者， 皮肤疾病将在UM-SBDRC内建立两个资源核心： 皮肤研究的效率和转化影响;提供独特的资源和专业知识; 促进新的发现;并提供有关实验设计和分析的培训和指导。在 此外，资源中心将不断创新，以保持我们的研究在该领域的前沿。 功能分析核心将为CRISPR/Cas9提供独特、创新、最先进的服务。 角化细胞中的诱变，以及遗传改变的角化细胞的转录组学和表观基因组学分析， 并集成到系统生物学管道中进行数据分析和解释。该核心还将提供 UM-SBDRC成员直接访问我们的生物信息学资源和大规模基因组和 人类皮肤病的转录组学数据集通过一个可搜索的基于网络的界面。动物 建模核心将提供设计，开发，验证和表征服务， 基因工程小鼠模型（GEMM），以帮助功能验证中产生的结果， 功能分析核心。动物模型核心还将为GEMM提供资源和指导 表型分析和体内操作，包括转基因诱导方案、UV照射、炎症和 纤维化模型，以及从GEMM建立细胞培养物。行政核心将提供 领导，监督和UM-SBDRC的结构，并建立和管理一个强大的丰富 计划，其中包括指导计划，试点和可行性计划，旅行奖励，客座演讲者 系列，和年度研讨会，与强大的机构和部门的支持中心。在 总之，UM-SBDRC将提供先进的基础设施和最先进的资源、专业知识， 密歇根州的培训机会。这个新中心符合NIAMS的使命， 研究基础设施，为进行研究的研究人员提供的共用设施、服务和资源 皮肤生物学和疾病的研究。UM-SBDRC将丰富和提高正在进行的基础和 转化研究，帮助外部调查人员进入皮肤生物学领域，扩大范围， 皮肤研究，并加速新的发现，最终造福于各种皮肤疾病的患者。

PROJECT SUMMARY - OVERALL The overall goal of the University of Michigan Skin Biology and Diseases Resource-based Center (UM- SBDRC) is to leverage our strengths in genomic editing, bioinformatics and mouse modelling to enable rapid transition from discovery to function, address biological and disease context, and facilitate development of appropriate state-of-the-art in silico approaches and in vivo mouse models of disease. A major emphasis of the UM-SBDRC is to apply years of cutting-edge mouse modeling expertise to the study of inflammatory skin disease using new knowledge gained through big-data approaches. Thus, the UM-SBDRC will both facilitate and accelerate ongoing research towards new discoveries and ultimately benefit a wide range of patients with cutaneous disorders. Two Resource Cores will be established within the UM-SBDRC: they will enhance efficiency and translational impact of cutaneous research; provide access to unique resources and expertise; facilitate new discoveries; and provide training and guidance regarding experimental design and analysis. In addition, the Resource Cores will continuously innovate to maintain our research at the forefront of the field. The Functional Analytics Core will provide unique and innovative, state-of-the art services for CRISPR/Cas9 mutagenesis in keratinocytes, with transcriptomic and epigenomic profiling of genetically-altered keratinocytes, and integration into a systems biology pipeline for data analysis and interpretation. This core will also provide UM-SBDRC members direct access to our bioinformatics resources and to large-scale genomic and transcriptomic datasets of human skin disease through a searchable web-based interface. The Animal Modeling Core will provide services for the design, development, validation, and characterization of genetically-engineered mouse models (GEMMs) to aid in the functional validation of results generated in the Functional Analytics Core. The Animal Modeling Core will also provide resources and guidance for GEMM phenotyping and in vivo manipulation, including transgene induction protocols, UV irradiation, inflammation and fibrosis models, and for establishment of cell cultures from GEMMs. The Administrative Core will provide leadership, oversight and structure for the UM-SBDRC and build and administer a robust Enrichment Program, which includes a Mentoring Program, Pilot & Feasibility Program, Travel Awards, Visiting Speaker Series, and an Annual Symposium, with strong institutional and departmental support for the Center. In summary, UM-SBDRC will provide sophisticated infrastructure and state-of-the-art resources, expertise, and training opportunities at Michigan. This new Center is in line with the NIAMS mission to provide critical research infrastructure, pooled facilities, services and resources to groups of investigators conducting research on skin biology and diseases. The UM-SBDRC will enrich and enhance effectiveness of ongoing basic and translational research, help bring outside investigators into the field of cutaneous biology, broaden the scope of skin research, and accelerate new discoveries to ultimately benefit patients with a wide range of skin diseases.