Next-Generation Genomic Imaging Technology

单细胞基因组学通过实现无偏见的分子生物学改变了生物学 全套细胞类型的鉴定和表征 组织。这种分子信息之间的“联姻”，包括一种 每个细胞类别表达的所有配体和受体的清单，以及 随附的有关它们的3D空间信息将使全面的 在给定的组织内重建活跃的信号中心。在这里，由 继续生物学家和分子生物化学家之间卓有成效的合作， 我们计划为3D多维提供一种变革性的新技术 在完整的人体组织中进行分子成像。我们方法的一个主要优势是 我们完全绕过了荧光成像的内在局限性，即 内源性自发荧光，低信噪比，限制在5个或更少 数据通道，通过利用镧系元素的共振能量传递。通过这样做，我们将 同时克服在检测单个信号和 大幅提高吞吐量。此外，我们提供的技术将是 兼具生物学家的友好性和低成本，提供了一种广泛可用和实用的 这一平台将迅速被世界各地的科学家广泛采用。

Single cell genomics has transformed biology by enabling unbiased molecular identification and characterization of the full panoply of cell types that make up a tissue. A 'marriage' between this molecular information, which includes an inventory of all the ligands and receptors expressed by each cell class, and the accompanying 3D spatial information about them will enable the comprehensive reconstruction of the active signaling centers within a given tissue. Here, by continuing a fruitful collaboration between a biologist and molecular biochemist, we propose to deliver a transformative new technology for 3D multi-dimensional molecular imaging in intact human tissue. A major strength of our approach is that we entirely bypass the intrinsic limitations of fluorescent imaging, namely endogenous autofluorescence, low signal to noise, and restriction to five or fewer data channels, by using lanthanide resonant energy transfer. By so doing, we will simultaneously overcome challenges in detection of individual signals and massively increase throughput. Furthermore, the technology we deliver will be both biologist 'friendly' and low cost, providing a widely available and practical platform that will be rapidly and broadly adopted by scientists worldwide.