Neuroanalytical Core

摘要-神经分析核心 神经分析核心开发和应用方法来测量体内的电活动神经递质。 将岩芯开发出来，然后用电化学方法轻松地测量绝对浓度 体内氧化的神经递质--这是其他方法不可能做到的。快速变化的世界 神经递质的细胞外浓度可由时相神经元放电引起。出于这个原因，化学品 传感器应该能够在广泛的时间范围内工作。一种理想的检测传感器 神经递质具有很高的灵敏度，可以区分化合物，并且具有快速的反应时间。 电化学方法为容易氧化的神经递质提供了一种方法，通过使用 神经递质释放部位旁边的电极。核心可以监控快速、阶段性的变化 FSCV或使用FSCAV的缓慢、紧张性变化。同时测定多巴胺和5-羟色胺 有可能进一步了解两个系统之间的相互作用。它 值得注意的是，活体实验将需要对大脑感兴趣的区域进行知情选择。 该仪器可用于多巴胺能和5-羟色胺能相互依赖的研究。 系统及其在学习和疾病状态中所起的作用。令人兴奋的是，这个核心被设计成允许 需要采用的其他方法。这个核心可以用来进一步发展行为神经科学和我们目前的 了解神经化学系统之间的联系。我们将方法论与神经科学相结合 确保我们将继续推进NIDA研究界的影响。

Summary – Neuroanalytial Core The Neuroanalytical Core develops and applies methods to measure electroactive neurotransmitters in vivo. The core will develop and then use electrochemical methods to measure the absolute concentrations of easily oxidized neurotransmitters in vivo – something not possible with other methodology. Fast changes in the extracellular concentration of neurotransmitters can arise from phasic neuronal firing. For this reason, chemical sensors should be able to operate on a wide range of time scales. An ideal sensor for the detection of neurotransmitters has high sensitivity, can distinguish between compounds, and has a fast response time. Electrochemical approaches offer a way to accomplish this for easily oxidized neurotransmitters by using an electrode next to sites where the neurotransmitter is released. The core can monitor rapid, phasic changes using FSCV or slow, tonic changes using FSCAV. Making simultaneous measurements of dopamine and serotonin on multiple timescales has the potential to further understanding of the interactions between the two systems. It is important to note that in vivo experimentation will require informed selection of the brain regions of interest. This instrument allows for the investigation of the interdependence of the dopaminergic and serotonergic systems and the role they play in learning and disease states. Excitingly, this core has been designed to allow additional methods to be incorporated. This core can be used to further behavioral neuroscience and our current understanding of the links between neurochemical systems. We couple the methodologies to neuroscience to ensure that we will continue to advance the impact of the NIDA research community.