Core C

项目摘要/摘要-核心C 体外电生理核心(Core C)将为体外实验提供服务 使用光遗传学、设计者受体等新工具进行电生理学研究 由特制药物(DREADD)和体外GCaMP6钙独占激活 成像。我们将准备脑片，进行膜片钳记录，优化 光发生和化学发生实验的方案，执行钙离子成像 在体外研究脑片，并传导通道视紫红质2(ChR2)辅助回路 映射(CRACM)。核心还将对记录的切片进行组织学检查，以及 分析关联数据。项目1(Saper)、项目2(Scammell)、项目3(Fuller) 而项目4(Vanderhost和Dymecki)将使用这个核心设施。这些项目是 不具备进行体外电生理记录的能力，并且没有 能够提供这项服务的机构资源。这些项目将受益于 核心的专业知识和共享资源。

Project Summary/Abstract- Core C The in vitro Electrophysiology Core (Core C) will provide services for in vitro electrophysiological studies using novel tools such as optogenetics, designer receptors exclusively activated by designer drugs (DREADDs) and in vitro GCaMP6 calcium imaging. We will prepare brain slices, conduct patch-clamp recordings, optimize the protocols for the optogenetic and chemogenetic experiments, perform Ca2+ imaging studies in in vitro slices, and conduct channelrhodopsin2 (ChR2)-assisted circuit mapping (CRACM). The Core will also perform histology of the recorded slices, and analyze the associated data. Project 1 (Saper), Project 2 (Scammell), Project 3 (Fuller) and Project 4 (Vanderhost and Dymecki) will use this Core facility. These projects are not equipped to conduct in vitro electrophysiology recordings and there are no institutional resources that can provide this service. The Projects will benefit from the expertise and shared resources of the Core.