Critical Resources Provided by UNMC RAP Biorepository Stimulate Cancer Research

项目摘要 高度创新的快速尸检计划(RAP)解决了胰腺和前列腺的主要限制 癌症研究：缺乏代表疾病所有阶段的高质量人类癌症标本。这 由保罗·M·格兰杰内特博士领导的计划，包括快速收集大量所有器官和 死亡5小时内的液体，是世界上独一无二的计划，旨在保持细胞和 组织的分子完整性。RAP是内布拉斯加大学医学中心的核心组成部分 (UNMC)胰腺癌研究计划和GU肿瘤学焦点小组，其任务是 收集、存档和分发近期胰腺癌患者的组织和体液 或者前列腺癌。弗雷德和帕梅拉医院胰腺癌研究项目的单位主任 巴菲特癌症中心是Michael A.Hollingsworth博士，而前列腺癌小组由Surinder Batra博士和 肖娜·博伊尔博士。格兰德内特博士是霍林斯沃斯博士手下的研究副教授，是 RAP总监和本次研究专家奖的申请者。快速尸检程序运行 在UNMC的胰腺癌症研究方案内，该方案对以下方面很重要，目前部分资金来自： 一项胰腺癌孢子P50赠款；正在进行和即将进行的P01和U01赠款以及几笔R01赠款，以 个人调查员。Grangenett博士亲自指导原发肿瘤的收集，残留未受影响 组织，远处转移，所有未受影响的组织，以及所有PDAC和 前列腺癌患者。他还建立了一个广泛的正常组织获取计划，该计划高度 被利用了。Grangenett博士预先确定了如何根据以下需要对特定样本进行优先排序 为赔偿委员会和其他机构的项目提供资金。目前，格兰杰内特博士向 UNMC和UNMC外的多个实验室对血液生物标记物(蛋白质、 RNA，DNA)，循环中的肿瘤细胞，患者衍生的模型，包括有机物和异种移植，以及其他 流程。剩余的样品被快速冷冻，在长期存储中维护并可用 通过请求和合作进行地方、国家和国际癌症研究。博士的关键角色。 Grangenett将协调30名说唱志愿者的培训和参与，并与 合作者确定他们的研究项目所需的样本，根据需要修改我们的SOP 获取这些样本，确认样本质量，然后将这些样本分发给调查人员。它是 对于这一资源的领导者来说，成为一名训练有素的研究专家是必不可少的， 通过参与尸检的经验，对患者样本可获得性的全面了解， 以及与每个研究人员在科学层面上互动的能力。格兰杰内特博士在这方面发挥了重要作用 这些生物标本的收集、加工、注释和分发的各个方面都起着至关重要的作用 在扩大与赔偿委员会调查人员以及在国家和国际一级的合作项目方面发挥作用。

Project Summary The highly innovative Rapid Autopsy Program (RAP) addresses a major limitation in pancreatic and prostate cancer research: the lack of quality human specimens of cancer representing all stages of disease. This program, led by Dr. Paul M. Grandgenett, which includes rapid collection of large quantities of all organs and fluids within 5 hours of death, is a unique program in the world and is designed to maintain cellular and molecular integrity of the tissue. The RAP is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program and the GU Oncology Focus Group, that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreas or prostate cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center is Dr. Michael A. Hollingsworth, while the prostate group is led by Dr. Surinder Batra and Dr. Shawna Boyle. Dr. Grandgenett, a Research Associate Professor under Dr. Hollingsworth's aegis, is the director of the RAP and the applicant for this Research Specialist Award. The rapid autopsy program operates within the Pancreatic Cancer Research Program at UNMC and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending P01 and U01 grants and several R01 grants to individual investigators. Dr. Grandgenett personally directs the collection of primary tumor, residual unaffected tissues, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites for all PDAC and Prostate cancer patients. He has also established an extensive normal tissue acquisition program that is highly utilized. Dr. Grandgenett determines in advance how specific samples will be prioritized based on the needs of funded projects both at UNMC and at other institutions. Currently, Dr. Grandgenett provides fresh samples to multiple laboratories, at UNMC and outside-UNMC, conducting research on blood-based biomarkers (proteins, RNA, DNA), circulating tumor cells, patient-derived modeling including organoids and xenografts, and other processes. The remaining samples are snap frozen, maintained in long-term storage and made available through request and collaboration for local, national and international cancer studies. Key roles of Dr. Grandgenett is to coordinate the training and participation of the thirty RAP volunteers and to work with collaborators to determine the samples necessary for their research project, to modify our SOP as needed to obtain those samples, confirm specimen quality and then to distribute those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of patient sample availability, as well as the ability to interact on a scientific level with each investigator. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens and plays a vital role in expanding collaborative projects with investigators at UNMC and on a national and international level.