CHEETAH Center for the Structural Biology of HIV Infection, Restriction, and Viral Dynamics

核心摘要 表达、纯化、鉴定和确定重组HIV相关蛋白、RNA和 他们的综合体是我们所有科学项目和核心成功的核心。我们的蛋白质生产，生物物理 表征和结构确定核心(核心3)旨在确保所有猎豹中心成员和 学员有机会接触到最先进的仪器和建筑设计、真核生物和细菌蛋白质方面的专业知识。 表达、生物物理表征、X射线结晶学、低温电子显微镜以及生物物理和结构生物学方面的培训。 核心3提供的服务将建立在我们在表达、描述和确定结构方面的成功历史之上 与艾滋病毒相关的蛋白质和复合体。 组成部分1(蛋白质和复合体的表达和纯化)将支持印度猎豹中心的实验室 蛋白质的生产需要获得对HIV-1生物学重要过程的机械性洞察，有可能 为开发新的治疗方法提供信息。所有相关资源都位于 犹他大学的生物化学。 组成部分2(生物物理特性)将定义和验证蛋白质和复合体的生物物理特性 由猎豹中心成员使用最先进的技术和仪器制作。这些数据将提供 机械洞察，并将支持和补充高分辨率结构研究。关联的指令插入是 在犹他大学生物化学系，以及实验设计、实施和数据 加工是与猎豹中心成员合作进行的。该组件还将执行密钥 培训在我们的访问学者计划中的作用。 构成部分3(结构确定)将通过确定蛋白质的结构来支持猎豹中心实验室 以及对HIV-1生物学很重要的复合体。结构将由单粒子低温电子显微镜或X射线确定 结晶学，从而为机械理解提供了基础，有可能为发展提供信息 新的治疗方法。这一部分还将在我们的访问学者计划中发挥关键的培训作用。

CORE SUMMARY The ability to express, purify, characterize, and determine the structures of recombinant HIV-related proteins, RNAs, and their complexes is central to the success of all of our Scientific Projects and Cores. Our Protein Production, Biophysical Characterization, and Structure Determination Core (Core 3) is designed to ensure that all CHEETAH Center members and trainees have access to state-of-the-art instrumentation and expertise in construct design, eukaryotic and bacterial protein expression, biophysical characterization, X-ray crystallography, cryoEM, and training in biophysics and structural biology. Services offered by Core 3 will build on our history of successes in expressing, characterizing, and determining the structures of HIV-related proteins and complexes. Component 1 (Expression and Purification of Proteins and Complexes) will support CHEETAH Center laboratories in the production of proteins needed to gain mechanistic insight into processes important for HIV-1 biology, with potential to inform the development of novel therapeutic approaches. All associated resources are located in the Department of Biochemistry at the University of Utah. Component 2 (Biophysical Characterization) will define and validate the biophysical properties of proteins and complexes produced by CHEETAH Center members using state-of-the-art techniques and instrumentation. These data will provide mechanistic insights, and will support and complement high-resolution structural studies. The associated instrumentation is housed in the Department of Biochemistry at the University of Utah, and experimental design, implementation, and data processing are conducted in collaboration with CHEETAH Center members. This Component will also perform a key training role in our Visiting Scholars Program. Component 3 (Structure Determination) will support CHEETAH Center laboratories by determining structures of proteins and complexes that are important for HIV-1 biology. Structures will be determined by single particle cryoEM or X-ray crystallography, thereby providing a foundation for mechanistic understanding with potential to inform the development of novel therapeutic approaches. This Component will also perform a key training role in our Visiting Scholars Program.