Impacts of Benzo[a]pyrene on Microbiome Development across Lifespan and Generations and the Behavioral Consequences

项目总结/摘要 这项补助金申请旨在支持黑人，女性，退伍军人的专业发展 正在从事毒理学研究科学家职业的研究生。为了实现这一目标， 候选人的共同导师--托马斯夏普顿博士和罗宾坦盖博士--与候选人一起工作， 制定一个研究，培训和指导计划，以确保候选人的专业成功。这 该计划包括一个广泛的跨学科培训计划，包括教学和体验 学习模式，以及个性化的，亲密的和支持性的导师计划。另夕h 计划包括一个跨学科和尖端的研究项目，将帮助候选人弹射他们的 职业前进。特别是，候选人将进行研究，将R 01父母奖扩展到 免费的，但新的领域。简而言之，父母奖的重点是定义如何早期生活暴露于 污染物影响肠道微生物组的组装，损害行为发育，并特别关注 苯并[a]芘作为模式污染物。作为补充补助金的一部分，候选人将应用这些工具， 根据母合同开发的技术，用于调查特定类别的环境化学品， 关注，即各种各样的全氟烷基和多氟烷基物质（PFAS），已被证明损害 脊椎动物的行为发展这样做，候选人将解决如何胚胎暴露于 各种PFAS影响肠道微生物组组装，以及这些肠道微生物组的改变是否介导 PFAS如何损害行为。因此，候选人将获得新兴的跨学科经验 毒理学领域，特别是在生物信息学和数据科学方法方面，并将澄清 潜在的机制，通过PFAS引起他们对脊椎动物的行为的影响。总体而言，拟议计划 将装备一个充满激情的年轻科学家从一个代表性不足的社区在竞争中茁壮成长， 研究景观。

PROJECT SUMMARY/ABSTRACT This supplement grant application aims to support the professional development of a Black, female, veteran graduate student who is pursuing a career as a toxicology research scientist. To accomplish this goal, the candidate’s co-mentors – Dr. Thomas Sharpton and Dr. Robyn Tanguay – have worked with the candidate to develop a research, training, and mentorship plan that will ensure the candidate’s professional success. This plan includes an extensive interdisciplinary training program that incorporates both didactic and experiential learning modalities, as well as a personalized, intimate, and supportive mentorship program. Additionally, the plan includes an interdisciplinary and cutting-edge research project that will help the candidate catapult their career forward. In particular, the candidate will conduct research that extends the R01 parent award into complimentary, but new areas. Briefly, the parent award focuses on defining how early life exposure to pollutants impacts assembly of the gut microbiome to impair behavior development, and focuses specifically on benzo[a]pyrene as a model pollutant. As part of this supplement grant, the candidate will apply the tools and technologies developed under the parent award to investigate a specific class of environmental chemicals of concern, namely a diverse array of per- and polyfluoroalkyl substances (PFAS) that have been shown to impair behavior development in vertebrates. In so doing, the candidate will resolve how embryonic exposure to various PFAS impacts gut microbiome assembly and whether these alterations to the gut microbiome mediate how PFAS impair behavior. As a result, the candidate will obtain interdisciplinary experience in an emerging area of toxicology, especially with respect to bioinformatic and data science approaches, and will clarify potential mechanisms through which PFAS elicit their effect on vertebrate behavior. In total, the proposed plan will equip a passionate junior scientist from an underrepresented community to thrive in the competitive research landscape.