Shared Resource 14: Proteomics (PSR)

项目摘要-蛋白质组学共享资源（PSR） PSR为蛋白质组学实验提供专业知识和最先进的仪器。的PSR 嵌入在OSU校园化学仪器中心，提供有竞争力的价格和卓越的 服务，利用慷慨的支持，从研究和学院办公室，并从NIH的赠款。 在上次审查中，PSR被评为分析共享资源组的杰出成员， 三个可寻址的评论，以减少周转时间，实际上由于 作为俄勒冈州立大学设施的非癌症用户的增加，以及感知到的重复服务。主要服务包括：1） 样品制备、MS实验设计、数据分析和时间表咨询; 2）蛋白质鉴定 在各种样品基质上使用最先进的MS; 3）深入蛋白质表征，包括但不限于 仅限于翻译后修饰、蛋白变体/突变、蛋白截短位点检测、替代 剪接形式检测、从头蛋白测序、蛋白交联和蛋白-蛋白相互作用; 4） 使用无标记（光谱计数和/或相对强度）或稳定同位素标记技术的蛋白质定量 （SILAC或ITRAQ/TMT）、定量MALDI组织成像和靶向质谱法;以及 BISR使用商业和内部开发的软件平台提供的分析。主要装备 在过去的5年里，通过三个NIH S10赠款和一个资助的P41资源中心， 方法开发PSR的具体目标是：1）提供先进的基于质谱的蛋白质组学 服务; 2）提供创新的蛋白质组学数据分析和生物信息学平台，以方便用户 口译; 3）提供实验设计咨询和操作MS仪器的培训。 在本供资周期内，方案支助和改革提供了关键服务，支持了44份出版物（5份> 10份影响 因子），380个用户和8个NCI赠款，包括1个K22，1个P01，4个R 01，1个R33和1个U 01。PSR对于以下方面至关重要： OSUCCC的研究重点是免疫肿瘤学，翻译基因组学，癌症工程和癌症 预防和生存。先进的分析平台使研究人员能够发现新的差异 在血清、尿液、BAL液、唾液、冷冻组织、福尔马林固定组织、细胞培养基中表达蛋白， 和细胞裂解物。鉴于OSUCCC的强劲招聘，对服务和新技术的需求将 增加海矿资源公司将在达到能力之前扩大其工作人员、仪器和服务。解决 为此，计划中的新服务正在实施或扩展（与BISR、毛细管 电泳和离子迁移率方法，组织成像和结构生物学使用天然MS，如开发的 P41资源）。在下一个资助期内，PSR将成为免疫监测的成员。 探索平台。PSR的年度预算为1，993，101美元，而CCSG的要求为158，381美元。因此，在本发明中， PSR利用了广泛的机构支持，仅寻求CCSG资金的7.9%支持。

PROJECT SUMMARY – PROTEOMICS SHARED RESOURCE (PSR) The PSR provides expertise and access to state-of-the-art instrumentation for proteomics experiments. The PSR is embedded within the OSU Campus Chemical Instrument Center, offering competitive pricing and exceptional service by leveraging generous support from the Office of Research and Colleges, and grants from the NIH. During the last review, the PSR was rated Outstanding as part of the Analytics Shared Resource Group, with three addressable comments to reduce turn-around time, a perceived drop in percent users actually due to an increase of non-cancer users as an OSU facility, and perceived duplicative services. Major services include: 1) consultation for sample preparation, MS experiment design, data analysis, and timelines; 2) protein identification using state-of-the-art MS on a variety of sample matrices; 3) in depth protein characterization including, but not limited to, post-translational modifications, protein variant/mutations, protein truncation sites detection, alternate splice form detection, de novo protein sequencing, protein cross-linking, and protein-protein interactions; 4) protein quantification using label-free (spectral counts and/or relative intensity) or stable isotope label techniques (SILAC or ITRAQ/TMT), quantitative MALDI tissue imaging, and targeted mass spectrometry; and 5) data analysis provided with the BISR using commercial and in-house developed software platforms. Major equipment has been enhanced in the past 5 years through three NIH S10 grants and a funded P41 Resource Center for methods development. PSR Specific Aims are to: 1) provide advanced mass spectrometry-based proteomics services; 2) provide innovative proteomic data analytics and bioinformatics platforms to facilitate user interpretation; and, 3) provide consultation on experimental design and training on self-operated MS instruments. Over the current funding cycle, the PSR provided key services in support of 44 publications (5 > 10 impact factor), 380 users, and 8 NCI grants, including 1 K22, 1 P01, 4 R01s, 1 R33 and 1 U01. The PSR is critical to the OSUCCC research priorities of immuno-oncology, translational genomics, cancer engineering and cancer prevention and survivorship. Advanced analytical platforms allow researchers to discover novel differentially expressed proteins in serum, urine, BAL fluid, saliva, frozen tissues, formalin fixed tissues, cell culture media, and cell lysates. Given the robust OSUCCC recruitment, demand for services and new technologies will increase. The GSR will expand its staff, instrumentation and services before capacity is reached. To address this, planned new services are being implemented or expanded (informatics services with BISR, capillary electrophoresis and ion mobility methods, tissue imaging, and structural biology using native MS, as developed by a P41 Resource grant). Over the next funding period, the PSR will be a member of the Immune Monitoring and Discovery Platform. The annual budget of the PSR is $1,993,101, yet the CCSG request is $158,381. Thus, the PSR leverages extensive institutional support and seeks only 7.9% support from CCSG funds.