HEXAMERIC SERUM PROTEIN EXPRESSION IN THE MOSQUITO

蚊子是世界范围内对人类的滋扰和健康危害， 其他动物。在美国，蚊子是一种重要的媒介 治疗多种不同的病毒性疾病，包括脑炎。AS 目前还没有有效的手段根除这种疾病 蚊子，确定新的蚊子控制策略是 显然有赖于更好地了解生物化学和 蚊子发育的遗传基础。 在这项拟议的研究中，六聚体的表达和潜在的作用 血清蛋白质在变态发育过程中的完成和建立 将在安第斯山脉的黄热病蚊子中进行成年生育能力的检查 埃及伊蚊。在全变态昆虫中，如蚊子，一组高度 丰富的血淋巴蛋白被称为六聚体或六聚体血清蛋白 (SPS)主要在幼虫的末期合成。 发展。建议的六聚体在变态过程中的作用包括 氨基酸的储存，用于成人结构的精致，成熟 成体角质层和血清中亲脂大分子的转运。缺勤 一种敌百虫的己聚氰菊酯与显著减少 生育能力。到目前为止，还没有发表过对六聚体SP的检查 在任何蚊子物种中都有表达。 将分析伊蚊六聚体SP的结构和命运，以及 特别关注亚单位组成、跨国改造后 和成体发育过程中组织的重新捕获。在隔离之后 以及伊蚊SP基因的克隆和DNA序列的特征 并将确定RNA合成的发展概况。 这些蚊子SP基因在果蝇中的表达将用于定位1) 昆虫典型六聚体SP亚基的功能域和2) 转录调控序列在组织-和阶段- 伊蚊LSP基因的比活性。克隆伊蚊的能力 拯救致命果蝇SP突变体的SP基因将作为一部分进行检测 一项关于六聚体SP在昆虫中的作用的更大规模的研究 蜕变。

Mosquitoes are a world-wide nuisance and health hazard to humans and other animals. In the United States the mosquito is an important vector for a number of different viral diseases, including encephalitides. As there is currently no efficient means for the eradication of the mosquito, the identification of new strategies for mosquito control is clearly dependent on a better understanding of the biochemical and genetic basis for mosquito development. In this proposed study, the expression and potential role of hexameric serum proteins in the completion of metamorphosis and the establishment of adult fertility will be examined in the yellow fever mosquito, Andes aegypti. In holometabolous insects such as the mosquito, a set of highly abundant hemolymph proteins termed hexamerins or hexameric serum proteins (SPs) is primarily synthesized during the last stage of larval development. Proposed roles for hexamerins during metamorphosis include amino acid storage for elaboration of adult structures, maturation of the adult cuticle and serum transport of lipophilic macromolecules. Absence of one dipteran hexmerin has been correlated with significantly reduced fertility. To date there is no published examination of hexameric SP expression in any mosquito species. The structure and fate of the Aedes hexameric SPs will be analyzed, with specific focus on subunit composition, post-transnational modification and tissue recapture during adult development. Following the isolation and characterization of clones for the Aedes SP genes, the DNA sequence and developmental profile of RNA synthesis will be determined. Expression of these mosquito SP genes in Drosophila will serve to map 1) functional domain of typical insect hexameric SP subunits and 2) transcriptional regulatory sequences underlying tissue-- and stage- specific activity of the Aedes LSP genes. The ability of cloned Aedes SP genes to rescue lethal Drosophila SP mutants will be examined as part of a larger study of the roles of hexameric SPs during insect metamorphosis.